Main description:

This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 2 delves into techniques for heterogeneous conjugates, protein fragment-complementation assays, BRET-based imaging, as well as instrumentation and software. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 2 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Contents:

Part I: Heterogeneous Conjugates

1. Quantitative Assessment of the Efficacy of Near-Infrared Photoimmunotherapy with Bioluminescence Imaging

Ryuhei Okada, Aki Furusawa, Peter L. Choyke, and Hisataka Kobayashi

2. Evaluation of the Efficacy of Saracatinib-Loaded Nanoparticles in Lymphatic Metastases of HNSCC with the Aid of Bioluminescence Imaging

Liwei Lang and Yong Teng

3. Reactive Oxygen Species-Responsive and Self-Illuminating Nanoparticles for Inflammation and Tumor Imaging

Xiaoqiu Xu, Qi Li, and Jianxiang Zhang

4. Antibacterial Activity Evaluation of ZnO, CuO, and TiO2 Nanoparticles in Solution and Thin Films

Christine Mielcarek, Rania Dadi, Anne Roynette, Alex Lemarchand, Andrei Kanaev, Karim Senni, Mamadou Traore, and Rabah Azouani

5. BRET-Based Dual-Color (Visible/Near-Infrared) Molecular Imaging Using a Quantum Dot/EGFP-Luciferase Conjugate

Setsuko Tsuboi and Takashi Jin

6. Polyhistidine-Tag-Enabled Conjugation of Quantum Dots and Enzymes to DNA Nanostructures

Christopher M. Green, Divita Mathur, Kimihiro Susumu, Eunkeu Oh, Igor L. Medintz, and Sebastian A. Diaz

7. Real-Time Quantification of Cell Internalization Kinetics by Bioluminescent Probes

Roxanne Castillo, Di Wu, Zheng Cao, Ran Yan, Kalea Fajardo, Jie Ren, Yunfeng Lu, and Jing Wen

Part II: Protein Fragment-Complementation Assays

8. Quantitative Imaging of Retinoic Acid Activities in Living Mammalian Cells

Sung-Bae Kim, Rika Fujii, and Ramasamy Paulmurugan

9. Bioluminescence-Based Complementation Assay to Correlate Conformational Changes in Membrane-Bound Complexes with Enzymatic Function

Sharon O'Neill and Ulla G. Knaus

10. The NanoBiT-Based Homogenous Ligand-Receptor Binding Assay

Ya-Li Liu and Zhan-Yun Guo

11. Monitoring Hippo Signaling Pathway Activity Using a Luciferase-Based Large Tumor Suppressor (LATS) Biosensor

Alexander Pipchuk and Xiaolong Yang

Part III: BRET-Based Imaging

12. Screening of Protein-Protein Interaction Modulators Using BRET-Based Technology

Lucia R. Fernandez, Jesica Mild, and Martin M. Edreira

13. TRUPATH: An Open-Source Biosensor Platform for Interrogating the GPCR Transducerome

Jeffrey F. DiBerto, Reid H.J. Olsen, and Bryan L. Roth

14. MERLIN: A BRET-Based Proximity Biosensor for Studying Mitochondria -ER Contact Sites

Hector Flores-Romero and Ana J. Garcia-Saez

15. Single Cell NanoBRET Imaging with Green-Range Halo-Tag Acceptor

Ovia Thirukkumaran and Hideaki Mizuno

16. Method for Measuring Bioactive Molecules in Blood by a Smartphone Using Bioluminescent Ratiometric Indicators

Mitsuru Hattori, Yukino Itoh, and Takeharu Nagai

17. BRET Sensors for Imaging Membrane Integrity of Microfluidically Generated Extracellular Vesicles

Ramasamy Paulmurugan, Yi Liu, Uday Kumar Sukumar, Masamitsu Kanada, and Tarik F. Massoud

18. In Vivo Assessment of Protein-Protein Interactions Using BRET Assay

Aaiyas Mujawar and Abhijit De

19. Live Cell Imaging of ATP Dynamics in Plant Cells

Ryoichi Sato and Shinji Masuda

20. Bioluminescence Resonance Energy Transfer for Global DNA Methylation Quantification

Natsumi Taka, Yuji Baba, Yuka Iwasaki, and Wataru Yoshida

21. In Vivo Bioluminescent Imaging of Bone Marrow Derived-Mesenchymal Stem Cells in Mice

Prakash Gangadaran, Ji Min Oh, Ramya Lakshmi Rajendran, and Byeong-Cheol Ahn

22. In Vivo Imaging of Oxidative and Hypoxic Stresses in Mice Model of Amyotrophic Lateral Sclerosis

Yasuyuki Ohta, Emi Nomura, Shinae Kizaka-Kondoh, and Koji Abe

Part IV: Instrumentation

23. ATP Sensing Paper with Smartphone Bioluminescence-Based Detection

Maria Maddalena Calabretta, Ruslan Alvarez-Diduk, Elisa Michelini, and Arben Merkoci

24. Organ Bioluminescence Imaging under Machine Perfusion Setting for Assessing Quality of Harvested Organ Preservation

Yuhei Higashi, Jun Homma, and Hidekazu Sekine

25. Time-Lapse Bioluminescence Imaging of Hes7 Expression In Vitro and Ex Vivo

Marina Sanaki-Matsumiya and Ryoichiro Kageyama

26. Bioluminescence-Optogenetics: A Practical Guide

Matthew A. Stern, Henry Skelton, Alejandra M. Fernandez, Claire-Anne N. Gutekunst, Ken Berglund, and Robert E. Gross

27. Applications of Bioluminescence-Optogenetics in Rodent Models

Matthew A. Stern, Henry Skelton, Alejandra M. Fernandez, Claire-Anne N. Gutekunst, Robert E. Gross, and Ken Berglund

28. One-Channel Microsliding Luminometer for Quantifying Low Energy Bioluminescent Lights

Sung-Bae Kim and Ramasamy Paulmurugan

29. Compact Eight-Channel Light Sensing System for Bioassays

Sung-Bae Kim, Sharon Seiko Hori, Negar Sadeghipour, Uday Kumar Sukumar, and Ramasamy Paulmurugan

30. Characterization of Firefly Flashes at Various Temperatures in Different Wavelength Regions

Anurup Gohain Barua and Angana Goswami

31. Bioluminescent Monitoring of Circadian Rhythms in Isolated Mesophyll Cells of Arabidopsis at Single-Cell Level

Shunji Nakamura and Tokitaka Oyama

Part V: Software

32. Exploring Phylogenetic Relationships and Divergence Times of Bioluminescent Species Using Genomic and Transcriptomic Data

Danilo T. Amaral, Monique Romeiro-Brito, and Isabel A.S. Bonatelli