Main description:

This detailed collection explores recent advances in molecular imaging techniques involving bioluminescence, currently employed in biolaboratories around the world. Volume 1 delves into techniques for the establishment of luciferins and luciferases, basic in vitro and in vivo applications, as well as protocols on multiplex imaging platforms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Bioluminescence: Methods and Protocols, Fourth Edition, Volume 1 presents practical guidance for researchers and technical staff on how to proceed with bioluminescence studies in their laboratories.

Contents:

Part I: Establishment of Luciferins and Luciferases

1. Gene Cloning and Functional Analysis of the Luciferase from Luminous Syllids of the Genus Odontosyllis

Rie Yasuno, Yasuo Mitani, and Yoshihiro Ohmiya

2. Synthetic Coelenterazine Derivatives and Their Application for Bioluminescence Imaging

Tianyu Jiang and Minyong Li

3. Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging

Nobuo Kitada, Shojiro Maki, and Sung-Bae Kim

4. Biosynthesis-Inspired Deracemizative Production of D-Luciferin In Vitro by Combining Luciferase and Thioesterase

Kazuki Niwa and Dai-ichiro Kato

5. Production of Metridia Luciferase in Native Form by Oxidative Refolding from E. coli Inclusion Bodies

Svetlana V. Markova, Marina D. Larionova, and Eugene S. Vysotski

6. Production of Copepod Luciferases via Baculovirus Expression System

Marina D. Larionova, Svetlana V. Markova, and Eugene S. Vysotski

7. Molecular Tension Probe for In Vitro Bioassays

Sung-Bae Kim, Rika Fujii, Simon Miller, and Mikio Tanabe

Part II: Basic In Vitro Applications

8. Optimized Loop-Mediated Amplification (LAMP) Allows Single Copy Detection Using Bioluminescent Assay in Real Time (BART)

Patrick Hardinge

9. A Simple and Rapid Bioluminescence-Based Functional Assay of Organic Anion Transporter 1 as a d-Luciferin Transporter

Katsuhisa Inoue, Koki Sugiyama, and Takahito Furuya

10. A Simple Bioluminescent Assay for the Screening of Cytotoxic Molecules against the Intracellular Form of Leishmania infantum

Diego Benitez, Andrea Medeiros, Cristina Quiroga, and Marcelo A. Comini

11. A Simple, Robust, and Affordable Bioluminescent Assay for Drug Screening against Infective African Trypanosomes

Estefania Dibello, Marcelo A. Comini, and Diego Benitez

12. Imaging of Autonomous Bioluminescence Emission from Single Mammalian Cells

Carola Gregor

13. Rapid Single-Cell Detection of Beer-contaminating Lactic Acid Bacteria Using Bioluminescence/Rapid Microbe Detection

Toshihiro Takahashi and Yasukazu Nakakita

14. Bioluminescence of Aliivibrio fischeri in Artificial Seawater and Its Application in Fungicide Sensing

Hitomi Kuwahara and Hiroshi Morita

15. A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor

Thabat Khatib, Berndt Muller, and Peter McCaffery

16. Design of an Intron-Retained Bioluminescence Reporter and Its Application in Imaging of Pre-mRNA Splicing in Living Subjects

Fu Wang, Si Chen, Haifeng Zheng, and Bin Guo

17. Generation of Bi-Reporter Expressing Tri-Segmented Arenavirus

Chengjin Ye and Luis Martinez-Sobrido

18. Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2

Desarey Morales Vasquez, Kevin Chiem, Chengjin Ye, and Luis Martinez-Sobrido

19. Generation, Characterization, and Applications of Influenza A Reporter Viruses

Kevin Chiem, Aitor Nogales, and Luis Martinez-Sobrido

Part III: Basic In Vivo Applications

20. Optimized Aequorin Reconstitution Protocol to Visualize Calcium Ion Transients in the Heart of Transgenic Zebrafish Embryos In Vivo

Manuel Vicente, Jussep Salgado-Almario, Antonio Martinez-Sielva, Juan Llopis, and Beatriz Domingo

21. Quantification and Imaging of Exosomes via Luciferase-Fused Exosome Marker Proteins: ExoLuc System

Tomoya Hikita and Chitose Oneyama

22. Bioluminescent Tracking of Human Induced Pluripotent Stem Cells In Vitro and In Vivo

Toshinobu Nishimura, Kouta Niizuma, and Hiromitsu Nakauchi

23. Noninvasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase

Yang Bi, Nannan Zhang, and Yun He

24. Bioluminescence Imaging for Evaluation of Antitumor Effect In Vitro and In Vivo in Mice Xenografted Tumor Models

Kazuhide Sato

25. Detection of Spontaneous Bone Metastases of Solid Human Tumor Xenografts in Mice

Vera Labitzky, Ursula Valentiner, and Tobias Lange

26. In Vivo Imaging Analysis of an Inner Ear Drug Delivery in Mice: Comparison of Inner Ear Drug Concentrations Over Time

Sho Kanzaki, Shinsuke Shibata, Masaya Nakamura, Masahiro Ozaki, and Hideyuki Okano

27. Protocols for the Evaluation of a Lymphatic Drug Delivery System Combined with Bioluminescence to Treat Metastatic Lymph Nodes

Ariunbuyan Sukhbaatar and Tetsuya Kodama

28. In Vivo Bioluminescent Imaging of Rabies Virus Infection and Evaluation of Antiviral Drug

Kentaro Yamada and Akira Nishizono

29. Imaging Infection by Vector-Borne Protozoan Parasites Using Whole-Mouse Bioluminescence

Monica Sa, David Mendes Costa, and Joana Tavares

30. Longitudinal Tracing of Lyssavirus Infection in Mice via In Vivo Bioluminescence Imaging

Kate E. Mastraccio, Celeste Huaman, Eric D. Laing, Christopher C. Broder, and Brian C. Schaefer

Part IV: Multiplex Imaging Platforms

31. Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-Relapse Compounds

Annemarie M. Voorberg-van der Wel, Anne-Marie Zeeman, Ivonne G. Nieuwenhuis, Nicole M. van der Werff, and Clemens H. M. Kocken

32. Synthetic Assembly DNA Cloning of Multiplex Hextuple Luciferase Reporter Plasmids

Alejandro Sarrion-Perdigones, Yezabel Gonzalez, and Koen J.T. Venken

33. Multiplex Hextuple Luciferase Assaying

Alejandro Sarrion-Perdigones, Yezabel Gonzalez, Lyra Chang, Tatiana Gallego-Flores, Damian W. Young, and Koen J.T. Venken

34. Molecular Imaging of Tumor Progression and Angiogenesis by Dual Bioluminescence

Yue Liu, Ziyu Huang, and Zongjin Li