Main description:

Eukaryotic cells contain a plurality of organelles distinguished by their specific membranes and contents. Their biogenesis occurs by growth and division of preexisting structures rather than de novo. Mitochondria and chloroplasts, which appear to be descended from prokaryotic ancestors, have retained some DNA and the biosynthetic capability for its expression. They synthesize, however, only a few of their proteins themselves. Most of their proteins are synthesized on free ribosomes in the cytoplasm and are only assembled in the correct membrane after synthesis is complete. The biogenesis of peroxisomes and glyoxysomes also appears to occur by an incorporation of proteins synthesized first in the cytoplasm. Other organelles, the Golgi complex, lysosomes, secretory vesicles, and the plasma membrane, are formed in a different manner. Their proteins are assembled in the membrane of the endoplasmic reticulum during trans lation by bound ribosomes and they must then be transported to the correct membrane. The 1980 Mosbach Colloquium was one of the first attempts to discuss the biogenesis of the various organelles in biochemical terms. This was appropriate since the crucial problems now center on the search for signals and receptors that dictate the site of assembly, the route taken, and the final location of a particular organelle protein. The assembly of prokaryotic membranes and the membrane of an animal virus were also discussed, since these simpler systems might shed light on the biogenesis of organelles in eukaryotes.

Contents:

Structure and Nucleotide Sequence of the Cytochrome B Gene in Yeast Mitochondrial DNA.- Sequence of Mammalian Mitochondrial DNA.- The Optional Introns in Yeast Mitochondrial DNA.- Synthesis and Assembly of Mitochondrial Membrane Proteins.- Primary Structure of Mitochondrial Membrane Proteins: Evolutionary, Genetic and Functional Aspects.- Early Biological Evolution Derived from Chemical Structures.- Studies of the Maize Chloroplast Chromosome.- The Plastid Chromosomes of Several Dicotyledons.- Synthesis, Transport, and Assembly of Chloroplast Proteins.- Role of the Golgi Complex in Intracellular Transport.- The Role of Free and Membrane-Bound Polysomes in Organelle Biogenesis.- Transport of Membranes and Vesicle Contents During Exocytosis.- Assembly and Turnover of the Subsynaptic Membrane.- Biogenesis of Peroxisomes and the Peroxisome Reticulum Hypothesis.- Origin and Dynamics of Lysosomes.- The Semliki Forest Virus Envelope: A Probe for Studies of Plasma Membrane Structure and Assembly.- Assembly of Membrane Proteins in Escherichia coli. A Genetic Approach.- Studies of the Path of Assembly of Bacteriophage M13 Coat Protein Into the Escherichia coli Cytoplasmic Membrane.