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MORE ABOUT THIS BOOK
Main description:
This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Contents:
Part I: Introduction and Historical Background
1. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease
Ana Olivera and Juan Rivera
Part II: Obtaining the Cells: Purification, Culture, and Counting
2. Purification of Basophils from Peripheral Human Blood
Franco H. Falcone and Bernhard F. Gibbs
3. Mast Cell Purification Protocols
Jasmine Farrington, Elizabeth P Seward, and Peter T Peachell
4. Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells
Hans Jurgen Hoffmann
5. Generation and Culture of Peripheral CD34+ Stem Cell-derived Mast Cells (PSCMCs)
Yanyan Luo, Stefan Frischbutter, Joerg Scheffel, and Frank Siebenhaar
6. Modulating the Human Basophil Phenotype During its Development and Maturation: Basophils Derived from in vitro Cultures of CD34+ Progenitor Cells
Donald MacGlashan Jr.
7. Generation of Mast Cells from Murine Stem Cell Progenitors
Emily J. Swindle
8. Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model
Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc
9. The Absolute Basophil Count
Elena Borzova
Part III: Basophil/Mast Cell Lines
10. Mast Cell and Basophils Cell Lines: A Compendium
Egle Passante
11. Use of Humanized RBL Reporter Systems for Detection of Allergen-specific IgE Sensitization in Human Serum
Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J.C. Alcocer, and Franco H. Falcone
12. Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for Detection of Allergen-specific IgE in Patient Sera Using Allergen Microarrays
Marina Kalli, Marcos J. C. Alcocer, Andrew J. Blok, and Franco H. Falcone
13. NPY-mRFP Rat Basophil Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranulation
Nafal Barwary, Daniel Wan, and Franco H. Falcone
14. Use of Engineered Nanoparticles (ENPs) for the Study of High-affinity IgE Receptor Fc RI Receptor Engagement and Rat Basophil Leukemia (RBL) Cell degranulation
Alelwani W, Alharbi RA , Wan D , Vllasaliu D, Falcone FH , and Stolnik S
Part IV: Diagnostic Applications 15. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, and Didier G. Ebo
16. Basophil Activation Experiments in Immediate Drug Hypersensitivity: More Than a Diagnostic Aid
Didier G Ebo, Jessy Elst, Athina Van Gasse, Leander De Puysseleyr, Margaretha Faber, Margo M Hagendorens, Lina Mayorga, Christel Mertens , Chris H Bridts, Luc S De Clerck, and Vito Sabato
17. Basophil Activation Techniques: Staining of Exteriorized Granule Matrix Jessy Elst, Vito Sabato, Margo M Hagendorens, Michel van Houdt, Margaretha A. Faber, Chris H Bridts, Didier G Ebo, and Christel Mertens
18. Measurement and Functional Analysis of the Mas-related G protein-coupled receptor MRGPRX2 on Human Mast Cells and Basophils Jessy Elst, Vito Sabato, Margo M Hagendorens, Michel van Houdt, Margaretha A. Faber, Chris H Bridts, Didier G Ebo, and Christel Mertens
19. The Mast Cell Activation Test (MAT)
Rajia Bahri and Silvia Bulfone-Paus
Part V: Whole Cells: Functional Studies
20. Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique
Victor G. Bechmann, Anette Teo Hansen, Lars K. Poulsen, and Bettina M. Jensen
21. Measuring Histamine and Cytokine Release from Basophils and Mast Cells
Bettina M. Jensen, Ewa A. Bartko, Katrine Baumann, and Per S. Skov
22. Detection of Mast Cells and Basophils by Immunohistochemistry
Andrew F. Walls and Cornelia Amalinei
23. Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry
Lionel Rohner and Michaela Fux
24. Mast Cell Migration and Chemotaxis Assayed by Microscopy
Monika Bambouskova, Zuzana Rubikova, Lubica Draberova, Pavel Draber, and Petr Draber
25. Basophil Stimulation and Signaling Pathway
Edward F. Knol and Bernhard F. Gibbs
26. Gene Silencing Approaches in Mast Cells and Primary Human Basophils
Vadim V. Sumbayev and Bernhard F. Gibbs
27. Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells
Jose Mario Morgado, Laura Sanchez-Munoz, Cristina Teodosio, and Luis Escribano
Part VI: Whole Organism: Disease and Knockout Models
28. Mast Cell Mediated Reactions in vivo
Stefan Frischbutter, Joerg Scheffel, and Frank Siebenhaar
29. Identification of Murine Basophils by Flow Cytometry and Histology
Christian Schwartz and David Voehringer
PRODUCT DETAILS
Publisher: Springer (Springer-Verlag New York Inc.)
Publication date: August, 2020
Pages: 350
Weight: 947g
Availability: Available
Subcategories: Immunology