Main description:

The fundamentals of biochemistry and molecular biology are an important component of all disciplines of Biology. In the era of multidisciplinary approaches, the basic techniques in Biochemistry and Molecular Biology are much needed by the students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture, and allied subjects both at undergraduate and postgraduate levels. This book includes 15 chapters covering more than 135 experimental protocols. It discusses all the relevant topics like pH and buffers, spectrophotometry, chromatography, carbohydrates, lipids, proteins, electrophoresis, enzyme immunology, vitamins and pigments, metabolites, and molecular biology. It includes a wide variety of experiments ranging from preparation of culture media to PCR, Southern and Western blotting. All the experiments have been meticulously designed and special care has been given to the safety in the laboratory with precautions provided wheresoever required.

Contents:

General Introducton; pH and Buffers; Spectrophotometry; Centrifugation and Cell Fractionation; Chromatography; Carbohydrates; Lipids; Amino Acids and Proteins; Nucleic Acids; Electrophoresis; Immunology; Enzymes; Metabolites; Vitamins, Hormones and Pigments; Molecular Biology; Appendices; Readership: Students of Botany, Zoology, Microbiology, Biotechnology, Fisheries, Veterinary, Pharmacology, Physiology, Medicine, Genetics, Agriculture and allied subjects both at undergraduate and postgraduate levels.