Main description:

This volume covers a wide range of model systems from invertebrate to humans and in vitro and in vivo. Chapters detail methods on squid, aplysia, xenopus, mouse in vivo, ex vivo, rodent primary neurons, human-derived neurons, zebrafish, drosophila in vivo, primary neurons, c. elegans, and in vitro reconstitution assays.Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Axonal Transport: Methods and Protocols aims to further our understanding of the trafficking of cellular components in the nervous system leading to the advancement of basic knowledge that underlines the cell biology of the neuron.

The chapters " Live imaging of RNA Transport and Translation in Xenopus Retinal Axons, Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons, Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons, Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport, and In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport" are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com

Contents:

Part I: Squid, Aplysia, and Xenopus

1. The Giant Axon of the Squid: A Simple System for Axonal Transport Studies

Joseph DeGiorgis, Marcus Jang, and Elaine L Bearer

2. Live Imaging and Quantitative Analysis of Organelle Transport In Sensory Neurons of Aplysia Californica

Kerriann Badal, Yibo Zhao, Kyle Miller, and Sathyanarayanan Puthanveettil

3. Live imaging of RNA Transport and Translation in Xenopus Retinal Axons

Julie Qiaojin Lin and Jean-Michel Cioni

Part II: Mouse in vivo and ex vivo

4. Imaging Axonal Transport in Ex Vivo Central and Peripheral Nerves

Stacey Anne Gould, Robert Adalbert, Stefan Milde, and Michael Coleman

5. In vivo Imaging of Axonal Organelle Transport in the Mouse Brain

Johannes Knabbe, Jil Protzmann, and Thomas Kuner

6. Studying Axonal Transport in the Brain by Manganese-Enhanced Magnetic Resonance Imaging (MEMRI)

Elaine L. Bearer, Xiaowei Zhang,and Russell E. Jacobs

Part III: Rodent Primary Neurons

7. Microfluidic Neuromuscular Co-Culture System for Tracking Cell-To-Cell Transfer and Axonal Transport of Labeled Proteins

Ariel Ionescu and Eran Perlson

8. Imaging Diversity in Slow Axonal Transport

Archan Ganguly and Subhojit Roy

9. Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses

Wesley M. Tierney, Ian A. Vicino, Stella Y. Sun, Wah Chiu, Esteban A. Engel, Matthew P. Taylor, and Ian B. Hogue

10. Molecular Analysis of Axonal Transport Dynamics Upon Modulation of Microtubule Acetylation

Silvia Turchetto, Romain Le Bail, Loic Broix, and Laurent Nguyen

11. Live-cell Imaging of RNA Transport In Axons Of Cultured Primary Neurons

Tabitha J. Hees and Angelika B. Harbauer

12. Visualizing Vesicle-Bound Kinesins in Cultured Hippocampal Neurons

Andrew Montgomery, Alex Garbouchian, and Marvin Bentley

13. Retrograde Axonal Transport of Neurotrophins in Basal Forebrain Cholinergic Neurons.

Arman Shekari and Margaret Fahnestock

14. Use of Microfluidics Chambers to Image Axonal Transport In Adult Sensory Neurons

Maria Fransiska Emily, Lokesh Agrawal, Paolo Barzaghi, Miki Otsuki, and Marco Terenzio

Part IV: Human-derived Neurons

15. High-resolution Imaging of Mitochondria and Mitochondrial Nucleoids in Differentiated SH-SY5Y Cells

Emily Annuario, Kristal Y-W Ng, and Alessio Vagnoni

16. Assessment of Mitochondrial Trafficking as A Surrogate of Fast Axonal Transport in Human Induced Pluripotent Stem Cell Derived Spinal Motor Neurons

Arpan R. Mehta, Siddharthan Chandran, and Bhuvaneish T. Selvaraj

Part V: Zebrafish

17. In vivo Live Imaging of Axonal Transport In Developing Zebrafish Axons

Melody Atkins, Jamile Hazan, and Coralie Fassier

18. Visualizing the Intracellular Trafficking in Zebrafish Mauthner Cells

Rongchen Huang, Yang Xu, Min Chen, Leiqing Yang, Xinliang Wang, Yueru Shen, Yubin Huang, Bing Hu

Part VI: Drosophila In Vivo, Ex Vivo, and Primary Neurons

19. Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves

William M. Saxton, Angeline Lim, and Inna Djagaeva

20. Detailed Imaging of Mitochondrial Transport and Precise Manipulation of Mitochondrial Function with Genetically-Encoded Photosensitizers in Adult Drosophila Neurons

Francesca Mattedi, George Chennell, and Alessio Vagnoni

21. Mitochondrial DNA Transport in Drosophila neurons

Joseph M. Bateman

22. Live imaging of Axonal Transport in the Adult Drosophila Central Nervous System

Wayne Robinson and Tanja A Godenschwege

23. Drosophila Primary Neuronal Cultures as a Useful Cellular Model to Study and Image Axonal Transport

Andre Voelzmann and Natalia Sanchez-Soriano

24. High-resolution Live-Imaging of Axonal RNP granules in Drosophila Pupal Brain Explants

Caroline Medioni, Jeshlee Vijayakumar, Anne Ephrussi, and Florence Besse

Part VII: C. elegans

25. Analyzing the Impact of Gene Mutations on Axonal Transport in Caenorhabditis elegans

Yuzu Anazawa and Shinsuke Niwa

26. Methods to Quantify and Relate Axonal Transport Defects to Changes In C. Elegans Behavior

Syed Nooruzuha Barmaver, Muniesh Muthaiyan Shanmugam, and Oliver Ingvar Wagner

27. Imaging Intracellular Trafficking in Neurons of C. elegans.

Sravanthi S P Nadiminti and Sandhya P Koushika

Part VIII: In vitro Reconstitution Assays

28. In vitro Reconstitution of Molecular Motor-Driven Mitochondrial Transport

Verena Henrichs, Jakub Rohlena, Marcus Braun, and Zdenek Lansky

29. In vitro Reconstitution of Kinesin-Based, Axonal mRNA Transport

Julia Grawenhoff, Sebastian Baumann, and Sebastian P. Maurer