Main description:

This detailed new edition compiles fully updated practical methodologies and approaches for diagnosticians and researchers working toward a better understanding of animal coronaviruses. The book explores vital techniques in virus isolation, diagnostic approaches via the detection of viral nucleic acids or proteins, serology, disease-targeted animal species models, and next-generation sequencing and bioinformatics. Written for the invaluable Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls.
Thorough and hands-on, Animal Coronaviruses, Second Edition will help readers design and carry out their increasingly important projects in clinical research, diagnostics, disease surveillance, and epidemiological study involving this family of viruses.

Contents:

Part I: Reviews

1. Animal Coronaviruses

Leyi Wang and Dongwan Yoo

2. Feline Coronavirus: Insights into the Pathogenesis and Diagnosis

Subarna Barua, Olivia Lockyear, Diane Delmain, and Chengming Wang

3. Animal Models for Studying COVID-19 Pathology and Infection

Kush Kumar Yadav and Scott P. Kenney

Part II: Virus Isolation

4. Isolation of Porcine Epidemic Diarrhea Virus from Clinical Samples

Qiuhong Wang

5. Isolation and Characterization of Porcine Epidemic Diarrhea Virus Using Suspension Vero Cells

Fei-Fei Ge, Hui-Ru Yu, De-Quan Yang, Xin Li, Hou-Bin Ju, Hai-Xiao Shen, Jian Liu, Li-Ping Shen, Hong-jin Zhao, and Jian Wang

6. Isolation and Propagation of Porcine Deltacoronavirus in Cell Culture and Embryonated Chicken Eggs

Hui Hu

7. Isolation and Propagation of Infectious Bronchitis Virus (Avian Coronavirus) in Chicken Embryonated Eggs

Alejandro Banda and Lifang Yan

Part III: Diagnostic Approaches by Detection of Viral Nucleic Acid or Protein

8. Real-Time Reverse Transcription Polymerase Chain Reaction for Simultaneous Detection of Bovine Coronavirus and Other Pathogens

Ailam Lim, Jennifer Godhardt-Cooper, and Kathy Toohey-Kurth

9. TaqMan Real-Time RT-PCR Detection of Porcine Epidemic Diarrhea Virus

Shijuan Dong, Zhen Li, Ruisong Yu, Bingqing Chen, Fusheng Si, and Chunfang Xie

10. Real-Time RT-PCR for Detection of Feline Coronavirus

Leyi Wang, Colleen Olmstead, and Richard Fredrickson

11. SARS-CoV-2 Real-Time RT-PCR Assay in Animals

Leyi Wang, Colleen Olmstead, Karen Terio, and Richard Fredrickson

12. Real-Time PCR and Antigen-Capture ELISA for the Diagnosis of Porcine Deltacoronavirus Infection

Shaopo Zu, Jin Yuan, Xiaohui Jin, and Hui Hu

Part IV: Serology

13. Identification of a Neutralizing Epitope-Containing Segment in Turkey Coronavirus Spike Protein for Diagnostic Serology

Yi-Ning Chen, Ching Ching Wu, and Tsang Long Lin

14. Antibody-Capture Enzyme-Linked Immunosorbent Assay for Detecting Protective Antibodies to Turkey Coronavirus Based on Recombinant Spike Protein Segment with Neutralizing Epitope

Yi-Ning Chen, Mohamed Abdelwahab, Chien Chang Loa, Ming-Kun Hsieh, Ching Ching Wu, and Tsang Long Lin

15. ELISA, Virus Neutralization, and Immunofluorescence Assays for Detection of Antibodies Against Porcine Deltacoronavirus

Lu Xia, Yue Zhang, and Hui Hu

16. Luminescent Immunoprecipitation System (LIPS) Development for the Specific Detection of PDCoV and SARS-CoV-2 Antibodies

Patricia A. Boley and Scott P. Kenney

17. Construction of a Mouse-Feline Chimeric Neutralizing Antibody Against SARS-CoV-2

Fangfeng Yuan, Jin Cui, Xingyu Yan, and Ying Fang

18. Microneutralization Test of Avian Infectious Bronchitis Virus Using Vero Cells

Sheng Zhou, Yi Jiang, and Mengjun Tang

Part V: Disease-Targeted Animal Species as Animal Models

19. Pig Bioassay to Assess Infectivity of Porcine Epidemic Diarrhea Virus Detected in Various Sample Matrices

Jianqiang Zhang

20. Diagnostic Investigation and Confirmation of Emerging Turkey Coronaviral Enteritis

Yi-Ning Chen, Wan-Jung Chen, Ching Ching Wu, and Tsang Long Lin

Part VI: Next-Generation Sequencing and Bioinformatics

21. Whole Genome Sequencing of Porcine Epidemic Diarrhea Virus and Porcine Deltacoronavirus

Ganwu Li, Ying Zheng, Leyi Wang, Yan Zhang, and Huigang Shen

22. Whole-Genome Sequencing of Avian Infectious Bronchitis Virus by iSeq100 Platform and MinION

Lifang Yan, Alejandro Banda, and Leyi Wang

23. Phylogenetic and Recombination Analysis of Animal Coronaviruses

Kai Huang, Huachen Zhu, and Yi Guan