Main description:

Advances in Enzymology and Related Areas of MolecularBiology is a seminal series in the field of biochemistry,offering researchers access to authoritative reviews of the latestdiscoveries in all areas of enzymology and molecular biology. Theselandmark volumes date back to 1941, providing an unrivaled view ofthe historical development of enzymology. The series offersresearchers the latest understanding of enzymes, their mechanisms,reactions and evolution, roles in complex biological processes, andtheir applications in both the laboratory and industry.

Each volume in the series features contributions from leadingpioneers and investigators in the field from around the world. Allarticles are carefully edited to ensure thoroughness, quality, andreadability.

Volume 77 focuses on the most recent advances in thefield of practical preparative enzymology. Enzymes have long beenrecognized and utilized for the preparation of specialty chemicals,and enzymatic resolution and desymmetrization are mainstays of thefine chemical industry. Today, some of the most intriguingenzymatic synthetic approaches involve the construction of complexpathways within cells to prepare molecules much more complex thanthose that can be reasonably developed in cell–free applications;this volume considers some of those processes. Koffas and coworkersprovide an overview of the construction of complex biosyntheticpathways for the preparation of a variety of both fine andcommodity chemicals, including biofuels. The development of highlyefficient pathways involves a combination of rational design and,increasingly, evolutionary approaches, and Schmidt–Dannert reviewsthe use of evolutionary strategies in synthetic biology.Glycosylation of myriad biologically active species impacts both invivo activity and ADME behavior. The addition of carbohydratemoieties to complex organic molecules represents a dauntingsynthetic challenge, and Williams and Thorson review the variousstrategies for enzymatic glycosylation of complex natural products.Finally, Pimkin and Markham review the structure, activity, andmechanism of inosine monophosphate dehydrogenase, a central enzymeof purine biosynthesis.

With its wide range of topics and long historical pedigree,Advances in Enzymology and Related Areas of MolecularBiology can be used not only by students and researchers inmolecular biology, biochemistry, and enzymology, but also by anyscientist interested in the discovery of an enzyme, its properties,and its applications.

Back cover:

Advances in Enzymology and Related Areas of MolecularBiology is a seminal series in the field of biochemistry,offering researchers access to authoritative reviews of the latestdiscoveries in all areas of enzymology and molecular biology. Theselandmark volumes date back to 1941, providing an unrivaled view ofthe historical development of enzymology. The series offersresearchers the latest understanding of enzymes, their mechanisms,reactions and evolution, roles in complex biological processes, andtheir applications in both the laboratory and industry.

Each volume in the series features contributions from leadingpioneers and investigators in the field from around the world. Allarticles are carefully edited to ensure thoroughness, quality, andreadability.

Volume 77 focuses on the most recent advances in thefield of practical preparative enzymology. Enzymes have long beenrecognized and utilized for the preparation of specialty chemicals,and enzymatic resolution and desymmetrization are mainstays of thefine chemical industry. Today, some of the most intriguingenzymatic synthetic approaches involve the construction of complexpathways within cells to prepare molecules much more complex thanthose that can be reasonably developed in cell–free applications;this volume considers some of those processes. Koffas and coworkersprovide an overview of the construction of complex biosyntheticpathways for the preparation of a variety of both fine andcommodity chemicals, including biofuels. The development of highlyefficient pathways involves a combination of rational design and,increasingly, evolutionary approaches, and Schmidt–Dannert reviewsthe use of evolutionary strategies in synthetic biology.Glycosylation of myriad biologically active species impacts both invivo activity and ADME behavior. The addition of carbohydratemoieties to complex organic molecules represents a dauntingsynthetic challenge, and Williams and Thorson review the variousstrategies for enzymatic glycosylation of complex natural products.Finally, Pimkin and Markham review the structure, activity, andmechanism of inosine monophosphate dehydrogenase, a central enzymeof purine biosynthesis.

With its wide range of topics and long historical pedigree,Advances in Enzymology and Related Areas of MolecularBiology can be used not only by students and researchers inmolecular biology, biochemistry, and enzymology, but also by anyscientist interested in the discovery of an enzyme, its properties,and its applications.

Contents:

Contributors.

Preface.

Structure and Mechanism of RND–Type Multidrug Efflux Pumps(Hiroshi Nikaido).

Efflux Pumps of Gram–Negative Bacteria: Genetic Responses toStress and the Modulation of Their Activity by pH, Inhibitors, andPhenothiazines (Leonard Amaral, Seamus Fanning, and Jean–MariePagès).

Efflux Pumps of the Resistance Nodulation DivisionFamily: A Perspective of Their Structure, Function, and Regulationin Gram–Negative Bacteria (Mathew D. Routh, Yaramah Zalucki,Chih–Chia Su, Feng Long, Qijing Zhang, William M. Shafer, andEdward W. Yu).

The MFS Efflux Proteins of Gram–Positive and Gram–NegativeBacteria (Massoud Saidijam, Kim E. Bettaney, Dong Leng, PikyeeMa, Zhiqiang Xu, Jeffrey N. Keen, Nicholas G. Rutherford, AlisonWard, Peter J. F. Henderson, Gerda Szakonyi, Qinghu Ren, Ian T.Paulsen, Ingerid Nes, Jasmin K. Kroeger, and Anne–BritKolsto).

Efflux Pumps as an Important Mechanism for Quinolone Resistance(Jordi Vila, Anna Fabrega, Ignasi Roca, Alvaro Hernandez, andJose Luis Martinez).

Xenobiotic Efflux in Bacteria and Fungi: A Genomics Update(Ravi D. Barabote, Jose Thekkiniath, Richard E. Strauss,Govindsamy Vediyappan, Joe A. Fralick, and Michael J. SanFrancisco).

A Survey of Oxidative Paracatalytic Reactions Catalyzed byEnzymes That Generate Carbanionic Intermediates: Implications forROS Production, Cancer Etiology, and Neurodegenerative Diseases(Victoria I. Bunik, John V. Schloss, John T. Pinto, NataliaDudareva, and Arthur J. L. Cooper).

Author Index.

Subject Index.