Main description:

This detailed volume serves clinicians and basic science researchers studying the increasingly antibiotic resistant Gram-negative bacterium Acinetobacter baumannii. Chapters detail microbiological techniques, biochemical techniques, clinical samples, and next generation omics techniques to characterize the organism at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Acinetobacter baumannii: Methods and Protocols aims to ensure successful results in the further study of this high priority area of antibiotic study.

Contents:

1. Specimen Collection, Processing, Culture, and Biochemical Identification of Acinetobacter spp.

Binesh Lal, Saranya Vijayakumar, Shalini Anandan, and Balaji Veeraraghavan

2. Genotyping of Acinetobacter baumannii in Nosocomial Outbreak and Surveillance

Saranya Vijayakumar, Balaji Veeraraghavan, Agila K Pragasam, and Yamuna Devi Bakthavachalam

3. Antimicrobial Susceptibility Testing Methods for Acinetobacter spp

Balaji Veeraraghavan, Saranya Vijayakumar, Agila K Pragasam, Yamuna Devi Bakthavachalam, and John A J Prakash

4. Methods to Evaluate Colistin Heteroresistance in Acinetobacter baumannii

Edgar X. Sherman, Jessie E. Wozniak, and David S. Weiss

5. Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper Supplemented Liquid Media and on Copper-Containing Surfaces

Caitlin L. Williams and D. Scott Merrell

6. A New Method for Determination of Minimum Biofilm Eradication Concentration for Accurate Antimicrobial Therapy

Nithin Sam, Raziya Fathima, and Balaji Veeraraghavan

7. Transformation of Acinetobacter baumannii: Electroporation

Mitchell G. Thompson and Suleyman Yildirim

8. Methods for Natural Transformation in Acinetobacter baumannii

Gottfried Wilharm and Evelyn Skiebe

9. Vesicle Mediated Gene Transfer in A. baumannii

Shweta Fulsundar, Sara Domingues and Kaare M. Nielsen

10. Targeted Gene Replacement in Acinetobacter baumannii

Indranil Biswas and Joshua Mettlach

11. RecET-Mediated Recombineering in Acinetobacter baumannii

Ashley T. Tucker, Matthew J. Powers, M. Stephen Trent, and Bryan W. Davies

12. Methods for Tn-seq Analysis in Acinetobacter baumannii

Larry A. Gallagher

13. Tn7-Based Single Copy Insertion Vectors for Acinetobacter baumannii

Kaleigh Ducas-Mowchun, P. Malaka De Silva, Rakesh Patidar, Herbert P. Schweizer, and Ayush Kumar

14. Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii

Sarah E. Anderson and Philip N. Rather

15. A Simple Static Biofilm Assay for Acinetobacter baumannii

Indranil Biswas and Joshua Mettlach

16. BioFluxTM 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth

Bertrand Naudin, Anja Heins, Stephane Pinhal, Emmanuelle De, and Marion Nico

17. In Vitro Motility Assays for Acinetobacter Species

Indranil Biswas, Alexandra Machen and Joshua Mettlach

18. Desiccation Tolerance Assays for Acinetobacter baumannii

Xun Wang, M. Stephen Trent, and Bryan W. Davies

19. Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure

Caitlin L. Williams, Heather M. Neu, Sarah L.J. Michel, and D. Scott Merrell

20. PBP Isolation and DD-Carboxypeptidase Assay

Shilpa Pal and Anindya S. Ghosh

21. Extraction and Visualization of Capsular Polysaccharide from Acinetobacter baumannii

Kyle A. Tipton and Philip N. Rather

22. Isolation of Lipid Cell Envelope Components from Acinetobacter baumannii

Matthew J. Powers, Carmen M. Herrera, Ashley T. Tucker, Bryan W. Davies, and M. Stephen Trent

23. Methods for Detecting N-acyl-homoserine Lactone Production in Acinetobacter baumannii

Aimee R. Paulk Tierney and Philip N. Rather

24. Isolation and Characterization of the Acinetobactin and Baumannoferrin Siderophores Produced by Acinetobacter baumannii

William F. Penwell and Luis A. Actis

25. Skin and Soft Tissue Models for Acinetobacter baumannii Infection

Daniel V. Zurawski, Jaideep Banerjee, Yonas A. Alamneh, Jonathan P. Shearer, and Samandra T. Demons

26. Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection

Lauren D. Palmer, Erin R. Green, Jessica R. Sheldon, and Eric P. Skaar

27. Computational Prediction of sRNA in Acinetobacter baumannii

Sankalp Arya, Vineet Dubey, Deepak Sen, Atin Sharma, and Ranjana Pathania

28. Global Metabolic Analyses of Acinetobacter baumannii

Mohd Hafidz Mahamad Maifiah, Tony Velkov, Darren J. Creek, and Jian Li

29. Reverse Vaccinology Approach to Potential Vaccine Candidates against Acinetobacter baumannii

Fatima Shahid, Shifa Tariq Ashraf, and Amjad Ali