Main description:

Statin is a potential antihypercholesterolemic agent, which inhibits 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the rate limiting enzyme of cholesterol biosynthesis. The beneficial effects of statin therapy, however, are not limited to patients with hypercholesterolemia. Increasing evidence suggest that an activity level of HMG-CoA reductase may affect various cellular functions such as DNA synthesis, cell proliferation, diverse signal transduction pathways leading to pathogenesis. The use of HMG-CoA reductase inhibitor in varying diseases is under investigation. This book is the most current hands-on book in the field of hepatocarcinogenesis. This book provides valuable coverage in hepatocellular carcinoma. It offers the latest techniques and approaches to conduct the hepatocarcinogenesis studies such as cell proliferation and differentiation. In the present study, the authors have evaluated the chemo-preventive potential of statins as the end point biomarker in a diethylnitrosamine-induced rat hepatocarcinogenesis model by morphometric analysis of precancerous lesions during neoplastic transformation of cells. Further, hepatic histopathological assessments of preneoplastic lesions are carried out and correlated with the morphometric findings. Expression of proliferating cell nuclear antigen (PCNA), Bromodeoxyuridine (BrdU) and metallothionein (MT) are studied by enzyme labeled immunochemistry.

Feature:

The book deals with noble use of statin for prevention and treatment of cancer and thus provides a platform for the researchers in cancer therapy

The text is well supported by color photographs of hepatic histopathology (morphometeric analysis) providing the evidence of cancer inhibitory effect of statin

The easy demonstration of the research methodology helps the researcher to learn quickly

Back cover:

Statin is a potential antihypercholesterolemic agent, which inhibits 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the rate limiting enzyme of cholesterol biosynthesis. The beneficial effects of statin therapy, however, are not limited to patients with hypercholesterolemia. Increasing evidence suggest that an activity level of HMG-CoA reductase may affect various cellular functions such as DNA synthesis, cell proliferation, diverse signal transduction pathways leading to pathogenesis. The use of HMG-CoA reductase inhibitor in varying diseases is under investigation. This book is the most current hands-on book in the field of hepatocarcinogenesis. This book provides valuable coverage in hepatocellular carcinoma. It offers the latest techniques and approaches to conduct the hepatocarcinogenesis studies such as cell proliferation and differentiation. In the present study, the authors have evaluated the chemo-preventive potential of statins as the end point biomarker in a diethylnitrosamine-induced rat hepatocarcinogenesis model by morphometric analysis of precancerous lesions during neoplastic transformation of cells. Further, hepatic histopathological assessments of preneoplastic lesions are carried out and correlated with the morphometric findings. Expression of proliferating cell nuclear antigen (PCNA), Bromodeoxyuridine (BrdU) and metallothionein (MT) are studied by enzyme labeled immunochemistry.

Contents:

1.Introduction.- 1.1.Carcinogenesis.- 1.2.Pathology of Liver Cancer.- 1.3.Cancer chemoprevention.- 1.4.Statin .- 1.5.Histopathological and Biochemical Assay.- 1.6.Placental Glutathione S-Transferase Positive Foci.- 1.7.Cell Proliferation.- 1.8.Metallothionein.- 1.9.Objectives of the Present Work.- 2.Materials and Methods.- 2.1.Hepatic Histomorphometry and Histochemistry.- 2.2.Expression of Placental Glutathione S Transferasepositive (GST-P).- 2.3.Cell Proliferation Assay. 2.4.Metallothionein.- 3.Results and Discussion.- 3.1.Effect of Statin on Body and Liver Weights in rat treated with DEN + PB.- 3.2.Effect of Statin on the Number and Size Distribution of Visible Hyperplastic Nodules in Rat Liver Treated with DEN+PB.- 3.3.Effect of Statin on Hepatic Histopathology.- 3.4.Effect of Statin on the Induction of GGT-Positive Foci in Rats Treated with DEN + PB .- 3.5.Effect of Statin on the Induction of PAS-Reaction and Alkaline Phosphatase in Rats Treated with DEN + PB.- 3.6.Effect of Statin on the impression of GST-P Positive Foci Rat Liver Treated with DEN + PB.- 3.7.Effect of Statin on DEN + PB induced PCNA expression in Rat Liver.- 3.8.Effect of Statin on DEN + PB induced BrdU-Li in the Rat Liver .- 3.9.Effect of Statin on DEN + PB induced Metallothionein overexpression.- 4.Summary and Conclusions.- 5.Bibliography.