Main description:

Prefaces tend to be platitudinous and unconducive to perusal. To this hardened Editor, the appearance of the book represents the end of a stamina-testing saga surpassing any past experience. Amongst the numerous authors - a notably eminent bevy - some were angelic and others suffered harassment to produce, amidst day-to-day pressures, an eventual article in the cause of receptor investigation; few took exception to the strong editing that their material underwent. The reader of this book will be interested rather in its aims and ba- ground.- Does it merit a n his bookshelf? The book is not a 'Proceedings', but has sponsored-meeting parentage. Wi th company support, notably from BetaHED Pharmaceuti cals of Indianapolis, the 8th International Subcellular Hethodology Forum was held in July 1982 at the University of Surrey in Guildford. The vigorous debates, partly on aspects such as hormonal receptors and drug targeting, then narrowed to Neuroreceptor Hethodology at a NATO Advanced Research Workshop, perforce set up at short notice. But 'Proceedings' are proverbially ephemeral material reflecting an array of solo performances, whereas this book is hopefully more like an orchestra's performance, of classical together with new material. Retrievability of receptor 'know-how' has been a key aim. Locations in the text, including comments and supplementary material (designated 'NC'), are thoroughly indexed, whilst for some aspects a 'Retrieval Key' (p. 545) should be used. Related to receptor methodo logy, receptor features and phenomena get due attention in the text.

Contents:

#A some Strategies for Receptors: Binding, Size and Isolation.- #A-1 Where do we stand on receptors today?.- #A-2 Strategy in receptor binding studies.- #A-3 Principles of receptor binding assays: the GABA receptor.- #A-4 Specificity criteria for receptor sites in binding studies.- #A-5 Target size analysis of cell surface receptors and adenylate cyclase.- #A-6 Determination of molecular weights of receptors, in solution and in situ.- #A-7 Development and purification of calmodulin andibodies: use in elucidating multiple functions of the protein in eukaryotic cells.- #A-8 Approaches to isolating minor membrane components using monoclonal antibodies, as in purifying 5?-nucleotidase.- #A-9 Receptor purification: a brief overview.- #A-10 Isolation of purified membranes and membranous cell components for receptor studies.- #NC(A) NOTES and COMMENTS related to the foregoing topics.- #NC(A)-1 Molecular genetics applied to receptor analysis.- #B Membranes and Associated Molecules and Processes, Including Hormonal Phenomena.- #B-1 Isolation and characterization of spiny- (clathrin)-coated membranes and vesicles from rodent Liver and, role in receptor-mediated processes.- #B-2 Hormone receptor -adenylate cyclase interaction: superpotency, superaffinity and desensitization phenomena.- #B-3 Analyzing the steps in coupling the opiate receptor to adenylate cyclase in neuroblastoma x glioma hybrid cells NG108-15.- #B-4 Architecture of plasma membrane hormone receptor-effector systems.- #B-5 Epithelial sodium channels: ligand binding techniques in intact tissues and homogenates.- #B-6 Role of internal membrane-located receptors in intracellular trafficking.- #B-7 Subcellular strategies for tracing receptor-mediated ligand uptake pathways in liver.- #B-8 Receptor-mediated transfer of proteins from blood to bile.- #B-9 Involvement of microtubules in insulin-receptor turnover in cardiac myocytes.- #NC(B) NOTES and COMMENTS related to the foregoing topics.- #NC(B)-1 Thyroid membrane NAD-glycohydrolase and ADP-ribosyl-transferase, possibly relevant to TSH action.- #NC(B)-2 Isolated adenylate cyclase and ?-adrenergic receptor.- #NC(B)-3 Insulin internalization as studied by subcellular fractionation and electron-microscopic radioautography.- #NC(B)-4 Synaptosomes and synaptic membranes: isolation and morphology.- #C Homing of Circulating Peptides, Proteins and Drug Carriers.- #C-1 Characterization of cell-surface receptors for calcitonin in hormone-responsive cancer cells.- #C-2 Binding sites for pituitary growth hormone and prolactin in the liver and mammary gland of the pregnant rabbit.- #C-3 Affinity labelling of the thyrotropin receptor.- #C-4 Affinity labelling techniques for low-abundance membrane components: peptide hormone receptors and the D-glucose transporter.- #C-5 A glucose-sensitive glycoprotein recognition system on rat hepatic endothelial cells.- #C-6 Plasma lipoproteins and the regulation of cellular function.- #C-7 Liposomes targeted to cellular receptors.- #C-8 Targeted drug carriers: biological activity of the N-acetylcysteine - liposome system.- #NC(C) NOTES and COMMENTS related to the foregoing topics.- #NC(C)-1 Hepatic growth hormone/prolactin receptors in male rats: intracellular localization, induction and internalization.- #NC(C)-2 The LDL receptor: functional studies and partial characterization.- #NC(C)-3 The receptor for immunoglobulin E on mast cells and basophils.- #NC(C)-4 Preparation of immunoliposomes.- #NC(C)-5 Targeting toxins by conjugation to antibodies: the effect of the linkage group on biological activity.- #NC(C)-6 Antibody-targeted liposomes: specific cell binding and cytotoxicity.- #NC(C)-7 Liposomes as vehicles for inhibitors of intracytoplasmic protein synthesis.- #D Cholinergic and Catecholamine Receptors.- #D-1 Examination of ligand occupation and the permeability response of the nicotinic acetylcholine receptor on intact cells.- #D-2 Group-specific reagents as probes for measuring agonist-mediated conformational changes of ?-adrenergic and muscarinic acetylcholine receptors.- #D-3 Physico-chemical studies of ?-adrenergic receptors on intact cells.- #D-4 In vitro regulation of ?-adrenergic receptors.- #D-5 Characterization and isolation of brain dopamine (D2) receptors.- #NC(D) NOTES and COMMENTS related to the foregoing topics.- #NC(D)-1 Affinity labelling of a cholinergic nicotinic receptor in cultured nerve cells.- #NC(D)-2 Solubilization and characterization of muscarinic acetylcholine receptors.- #NC(D)-3 Antibodies to a covalent antagonist used to isolate the muscarinic cholinergic receptor from rat brain.- #NC(D)-4 Myasthenia gravis - some experimental approaches.- #NC(D)-5 Guanine nucleotide regulation of agonist binding to hepatic ?1-adrenergic receptors.- #NC(D)-6 Biochemical and immunological methods to purify the ?-adrenergic receptors.- #E Various Neuroreceptors.- #E-1 Solubilization and characterization of active opiate binding sites.- #E-2 Selective tolerance development facilitates a differentiation of multiple opiate receptors.- #E-3 Subcellular fractionation of insect muscle.- #E-4 The receptor for ?-latrotoxin, studied in pre-synaptic membranes and membrane extracts.- #E-5 Allosteric interactions in receptor binding studies.- #NC(E) NOTES and COMMENTS related to the foregoing topics.- #NC(E)-1 A slowly dissociating form of bovine hippocampal synaptic membrane opiate receptors.- #NC(E)-2 Binding of reversible and irreversible ligands to brain opiate receptors.- #NC(E)-3 Affinity probes for opioid receptors in synaptosomes.- #NC(E)-4 Biochemical pharmacology of substance P receptors.- #NC(E)-5 Substance P-stimulated phosphatidylinositol breakdown in guinea pig ileum.- #NC(E)-6 Soluble GABA/benzodiazepine/barbiturate receptor interactions in mammalian brain.- #NC(E)-7 Characterization and purification of the glycine receptor of rat spinal cord.- #NC(E)-8 Observations on receptor sites for dihydropyridines.- #NC(E)-9 Specific high-affinity membrane receptors for biologically active phorbol and ingenol esters.- #NC(E)-10 Molecular graphics and computational chemistry techniques for drug design.- #NC(E)-RT Methodology for neuroreceptors Round-table discussions.- Computer-aided conformational approaches to modelling.- An Editor's Guide to receptor studies with cell-free systems.- Retrieval Key (topic index, complementing the General Index).- General Index.- Corrections to Vols. 11 & 12.