Main description:

The first edition of this book covered the basic treatment of the enzyme reaction using the overall reaction kinetics and stopped-flow method, the general properties of protein and cofactors, the control of enzyme reaction, and the preparation of enzyme protein. These topics are the basis of the enzyme research and thus suitable for the beginner in the field. The second edition presents the cofactors produced via post-translational modification of the enzyme's active site. These cofactors expand the function of enzymes and open a new research field. The carbonyl reagent phenylhydrazine and related compounds have been useful in finding some of the newly discovered cofactors and thus have been discussed in this edition. The topic of the control of enzyme activity through the channel of substrates and products in polyfunctional enzymes has also been expanded in this book.

Contents:

1. Overall Reaction Kinetics 2. Facors that Affect Enzyme Activity 3. Effect of pH, Temperature, High pressure on the Enzymatic Activity 4. Measurement of Individual Rate Constants 5. Structure of Protein 6. Cofactors 7. Search of Active site 8. Control of Enzyme Activity 9. Channeling of Substrates and Products
10. Preparation of Enzyme 11. A Case Study: L-Phenylalanine Oxidase (Deaminating and Decarboxylating)