Main description:

This Open Access volume provides a comprehensive overview of the latest tools available to scientists to study the many facets of whole-body regeneration (WBR). The chapters in this book are organized into six parts. Part One provides a historical overview on the study of the WBR phenomena focusing on the primary challenges of this research. Parts Two and Three explore a series of non-vertebrate zoological contexts that provide experimental models for WBR, showing how they can be approached with cellular tools. Parts Four, Five, and Six discuss the future advancements of WBR, reporting about the cutting-edge techniques in genetics and omics used to dissect the underlying mechanisms of WBR, and systems biology approaches to reach a synthetic view of WBR. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Whole-Body Regeneration: Methods and Protocols is a valuable resource for scientists and researchers who want to learn more about this important and developing field.

Contents:

Preface...Table of Contents...Contributing Authors...
Part I Historical Overviews
1. The Hazards of Regeneration: From Morgan's Legacy to Evo-DevoChiara Sinigaglia, Alexandre Alie, and Stefano Tiozzo
2. Studying Regeneration in Ascidians: An Historical OverviewVirginia Vanni, Loriano Ballarin, Fabio Gasparini, Anna Peronato, and Lucia Manni
Part II Zoological Approaches
3. Studying Protista WBR and Repair using Physarum polycephalumMegan M. Sperry, Nirosha J. Murugan, and Michael Levin
4. Studying Porifera WBR using the Calcerous Sponges LeucosoleniaAndrey I. Lavrov and Alexander V. Ereskovsky
5. Studying Ctenophora WBR using Mnemiopsis leidyi Julia Ramon-Mateu, Allison Edgar, Dorothy Mitchell, and Mark Q. Martindale
6. Studying Placozoa WBR in the Simplest Metazoan Animal, Trichoplax adhaerensHans-Jurgen Osigus, Michael Eitel, Karolin Horn, Kai Kamm, Jennifer Kosubek-Langer, Moritz Jonathan Schmidt, Heike Hadrys, and Bernd Schierwater
7. Collecting and Culturing Kamptozoans for Regenerative StudiesAchim Meyer, Julia Merkel, and Bernhard Lieb
8. Collecting and Culturing Bryozoans for Regenerative StudiesAbigail M. Smith, Peter B. Batson, Katerina Achilleos, and Yuta Tamberg
9. Studying Annelida Regeneration in a Novel Model Organism: The Freshwater Aeolosoma viride Chiao-Ping Chen, Sheridan Ke-Wing Fok, Cheng-Yi Chen, Fei-Man Hsu, Yu-Wen Hsieh, and Jiun-Hong Chen
10. Studying Annelida Body Regeneration under Environmental Stress in Diopatra neapolitanaAdilia Pires
11. Studying Annelida Regeneration using Platynereis dumeriliiMichel Vervoort and Eve Gazave
12. Collecting and Culturing Lineus sanguineus to Study Nemertea WBREduardo E. Zattara and Fernando A. Fernandez-Alvarez
13. Studying Xenacoelomorpha WBR using Isodiametra pulchra Bernhard Egger
14. Studying Echinodermata Arm Explant Regeneration using Echinaster sepositusCinzia Ferrario, Yousra Ben Khadra, Michela Sugni, M. Daniela Candia Carnevali, Pedro Martinez, and Francesco Bonasoro
15. Studying Hemichordata WBR using Ptychodera flavaAsuka Arimoto and Kuni Tagawa
16. Studying Tunicata WBR using Botrylloides ancepsArzu Karahan, Esra OEzturk, Berivan Temiz, and Simon Blanchoud
Part III Cellular Approaches
17. In Situ Hybridization to Identify Stem Cells in the Freshwater Sponge Ephydatia fluviatilisChiaki Kojima and Noriko Funayama
18. Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyiAbigail C. Dieter, Lauren E. Vandepas, and William E. Browne
19. Analysis of Spatial Gene Expression at the Cellular Level in Stony Corals Nikki Traylor-Knowles and Madison Emery
20. Studying Stem Cell Biology in Intact and Whole-Body Regenerating Hydra by Flow CytometryWanda Buzgariu, Jean-Pierre Aubry-Lachainaye, and Brigitte Galliot
21. Non-Invasive Intra-Vascular Micro-Transfusion in Colonial TunicatesLuis Albert Matas Serrato, Alessandro Bilella, and Simon Blanchoud
Part IV Genetic Approaches
22. Gene Manipulation in HydractiniaEleni Chrysostomou, Febrimarsa, Timothy DuBuc, and Uri Frank
23. Manipulation of Gene Activity in the Regenerative Model Sea Anemone, Nematostella vectensisEric M. Hill, Cheng-Yi Chen, Florencia del Viso, Lacey R. Ellington, Shuonan He, Ahmet Karabulut, Ariel Paulson, and Matthew C. Gibson
24. Monitoring Telomere Maintenance during Regeneration of AnnelidsNithila A. Joseph, Chi-Fan Chen, Jiun-Hong Chen, and Liuh-Yow Chen
25. Analysis of DNA Double Stranded Breaks using the COMET Assay in Planarians Paul G. Barghouth, Salvador Rojas, Lacey R. O'Dell, Andrew M. Betancourt, and Nestor J. Oviedo
26. Random Integration Transgenesis in a Free-Living Regenerative Flatworm Macrostomum lignanoJakub Wudarski, Kirill Ustyantsev, Filipa Reinoite, and Eugene Berezikov
27. RNAi Screening to Assess Tissue Regeneration in PlanariansRachel H. Roberts-Galbraith
28. Monitoring Chromatin Regulation in Planarians using Chromatin Immunoprecipitation followed by Sequencing (ChIP-seq)Divya Sridhar and Aziz Aboobaker
29. Assessing Chromatin Accessibility during WBR in AcoelsAndrew R. Gehrke and Mansi Srivastava
Part V OMICS Approaches
30. Single Cell Transcriptomic Analysis in the Regenerating Cnidarian Nematostella vectensisFlora Plessier, Sandrine Schmutz, Sophie Novault, and Heather Marlow
31. Characterization of Soluble Cell-Free Coelomic Fluid Proteome from the Starfish Marthasterias glacialisLaidson Paes Gomes, Catarina Gouveia e Silva, Jean-Charles Gaillard, Jean Armengaud, and Ana Varela Coelho
32. Using RNA-Sequencing for Transcriptome Profiling of Botrylloides sp. RegenerationMichael Meier and Megan J. Wilson
Part VI Integrative Approaches
33. Studying Mechanical Oscillations during Whole-Body Regeneration in HydraJaroslav Ferenc and Charisios D. Tsiairis
34. Combining RNAi-Mediated -catenin Inhibition and Reaggregation to Study Hydra Whole-Body RegenerationMatthias Christian Vogg and Brigitte Galliot
35. Creating a User-Friendly and Open-Access Gene Expression Database for Comparing Embryonic Development and Regeneration in Nematostelle vectensisOlivier Croce and Eric Roettinger
36. Formalizing Phenotypes of RegenerationDaniel Lobo
Subject Index List...