Main description:

It is perhaps not too much of an exaggeration to claim that experimental hematology as it flourishes today originated largely from the pioneering attempts to protect lethally radiated animals (1) by shielding of hemopoietic tissues by L. O. Jacobson (9), and (2) by treatment with bone marrow suspensions by E. Lorenz and his col- laborators (12). The site chosen for this annual meeting of the International Society for Experi- mental Hematology is given a special historic sig- nificance by the fact that it was 25 years ago that the first publication on this subject by Lorenz ap- peared from his laboratory at the National Insti- tutes of Health. Lorenz's discovery marked the beginning of a period which lasted until 1956, during which the protection afforded by hemopoietic cell suspensions was confirmed by many. This soon led to an intensive scientific de- bate on the mechanism of this protective effect: was it due to a humoral factor produced and pro- vided by the bone marrow-as Lorenz The Appearance of postulated-or to transplantation and subsequent proliferation of hemop- etic cells?
This question was defini- 1 the Multipotential tively answered in 1956 by evidence from three different laboratories (7, 15, 26), which demonstrated the origin of the cells Hemopoietic in the repopulated tissues using a variety of cellu- lar and immunologic markers. By the same token, these contributions marked the birth of radiation Stem Cell chimeras.

Contents:

I. Characterization of the Multipotential Stem Cell (CFU-s).- 1. The Appearance of the Multipotential Hemopoietic Stem Cell.- The spleen colony assay of rat HSC.- Concentration and identification of rat HSC.- Summary.- Acknowledgments.- References.- 2. An Immunologic Approach to Cell Cycle Analysis of the Stem Cell.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 3. Physical Separation of the Cycling and Noncycling Compartments of Murine Hemopoietic Stem Cells.- Theory.- Materials and methods.- Results and discussion.- Summary.- Acknowledgments.- References.- 4. Characterization of Bone Marrow and Lymph Node Repopulating Cells by Transplanting Mononuclear Cells into Radiated Dogs.- Experimental approach.- Results.- Discussion.- Summary.- Acknowledgments.- References.- II. Humoral and Cellular Control Agents.- 5. Concepts and Observations on the Regulation of Granulocyte Production.- Materials and methods.- Results.- Summary.- Acknowledgment.- References.- 6. The Regulatory Role of the Macrophage in Normal and Neoplastic Hemopoiesis.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 7. Humoral Regulation of Eosinophil Granulocytopoiesis.- Eosinophil granulocytopoiesis: A model system.- Production of humoral factor(s) in vitro.- Summary.- Acknowledgments.- References.- 8. Studies of the Erythroid Inductive Microenvironment in Vitro.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 9. In Vivo Colony Forming Unit Population Sizes in Hypertransfused Sl/Sld Mice.- Methods.- Results.- Discussion.- Summary.- References.- III. Physiology of Committed Stem Cells (CFU-e and CFU-m).- 10. Recognition of Hemopoietic Progenitors.- Summary.- References.- 11. Relationships between Early Hemopoietic Progenitor Cells Determined by Correlation Analysis of Their Numbers in Individual Spleen Colonies.- Experimental.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 12. Some Characteristics of in Vitro Erythroid Colony and Burst-Forming Units.- Methods and materials.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 13. Colony-Forming Unit, Megakaryocyte (CFU-m): Its Use in Elucidating the Kinetics and Humoral Control of the Megakaryocytic Committed Progenitor Cell Compartment.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgment.- References.- IV. Physiology of Committed Stem Cells (CFU-c).- 14. Negative and Positive Feedback Control of the Committed Granulocytic Stem Cell Compartment.- Physiologic control of the CFU-c.- Positive feedback system for CFU-c regulation.- Negative feedback system for CFU-c control.- Possible interaction of the two feedback systems in the regulation of the CFU-c compartment.- Summary.- References.- 15. Colony-Stimulating Factor and the Differentiation of Granulocytes and Macrophages.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 16. Characteristics of the in Vitro Monocyte-Macrophage Colony-Forming Cells Detected within Mouse Thymus and Lymph Nodes.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 17. Physical Characterization of a Subpopulation of Granulocyte/Monocyte Progenitor Cells (CFU-c).- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 18. Cell-Factor Interaction in Populations of Normal and Leukemic Blood Cells.- Results.- Discussion.- Summary.- Acknowledgments.- References.- V. Bone Marrow Transplantation Immunology.- 19. Bone Marrow Transplantation Immunology.- Summary.- Acknowledgments.- References.- 20. Modulation of Graft-versus-Host (GvH) Disease in the Rat; Effect of Hydroxyurea on the Mixed Lymphocyte Reaction and Graft-versus-Host Reactivity.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 21. Mechanism of Donor to Host Tolerance in Rat Bone Marrow Chimeras.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgment.- References.- 22. Effects of a Cell-Free Helper Factor(s) on the Kinetics of T-Cell Responses to Histocompatibility Antigens.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgment.- References.- VI. Experimental Models of Clinical Conditions in Hematology.- 23. The E(AkR)-Leukemia as Murine Model of Human Acute Lymphoid Leukemia for Immunotherapy Trials.- Summary.- References.- 24. Experimental Chemotherapy: A Rat Model for Human Acute Myeloid Leukemia.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 25. Serum Colony Stimulating Factor: A Marker for Graft-versus-Host Disease in Humans.- Methods.- Results.- Discussion.- Summary.- Acknowledgments.- Appendix: Case histories.- References.- 26. Changes in Human Bone Marrow Colony-Forming Cells following Chemotherapy Using an Agar Diffusion-Chamber Technique.- Methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.- 27. Graft-versus-Leukemia, Donor Selection for Adoptive Immunotherapy in Mice.- Materials and methods.- Results.- Discussion.- Summary.- Acknowledgments.- References.