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Laboratory Methods in Enzymology: Protein Part A
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Main description:

The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 530 volumes and 40,000 chapters in the collection, this is an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research, and genetics, just to name a few. This volume brings together a number of core protocols concentrating on protein, carefully written and edited by experts, including: Pulse-chase analysis to measure protein degradationLabeling a protein with fluorophores using NHS ester derivitizationImmunoaffinity purification of proteinsProteolytic affinity tag cleavagePurification of GST-tagged proteins


Practical Steady-state Enzyme KineticsQuantification of Protein Concentration using UV absorbance and Coomassie DyesPreparation of protein samples for mass spectrometry and N-terminal sequencingLysis of mammalian and Sf9 cellsIn Vivo [35 S]-Methionine IncorporationPulse-chase analysis to measure protein degradationLabeling of a Protein with Fluorophores using Maleimide DerivitizationLabeling a protein with fluorophores using NHS ester derivitizationProtein Derivitization-Expressed Protein LigationProtein BiotinylationPurification of His-tagged proteinsAffinity purification of a recombinant protein expressed as a fusion with the maltose-binding protein (MBP) tagImmunoaffinity purification of proteinsAffinity Purification of Protein Complexes Using TAP TagsStrep-tagged protein purificationProteolytic affinity tag cleavageAffinity Pull-down of Proteins using anti-FLAG M2 Agarose BeadsProtein Affinity Purification using Intein/Chitin Binding Protein TagsPurification of GST-tagged proteins


ISBN-13: 9780124200975
Publisher: Elsevier (Academic Press Inc)
Publication date: February, 2014
Pages: 514

Subcategories: Biochemistry, Diseases and Disorders, General Practice