Main description:

The development of synchrotron radiation (SR) as a research tool was driven largely by the needs of materials scientists and solid-state physi- cists. However, the availability of SR has extended significantly the capa bility of scientists who study biological structure with radiation. This volume contains some of the results reported at a symposium held at Brookhaven National Laboratory in May 1988 to discuss the application of synchrotron radiation to structural biology. We are grateful for financial support from the u. s. Department of Energy, the National Institutes of Health, Genentech, Inc. , Blake Indus- tries, Inc. , Evans and Sutherland Co. , The Upjohn Company, Eli Lilly and Company, Enraf-Nonius Service Corp. , and Associated Universities, Inc. We warmly thank Ms. Nancy Siemon for her tireless efforts with correspondence and the manuscripts for this symposium volume. Symposium Committee: Robert M. Sweet, Chair Malcolm S. Capel Benno P. Schoenborn John C. Sutherland Elizabeth C. Theil Stephen W. White Avril D. Woodhead Helen Z. Kondratuk, Coordinator v CONTENTS An Introduction to the Symposium ...1 R. M.
Sweet SYMPOSIUM LECTURE Developments in X-ray Technology and Their Contribution to Structural Biology ...3 H. E. Huxley SOME OF THE SYNCHROTRON FACILITIES FOR BIOLOGICAL STRUCTURAL STUDIES MacChess - A Macromolecular Diffraction Resource at the Cornell High Energy Synchrotron Source ...15 W. Schi1dkamp, K. Moffat, B. Batterman, D. Bilderback, T-Y. Teng, A. LeGrand and D. Szebenyi Facilities Available for Biophysics Research at the Stanford Synchrotron Radiation Laboratory...19 R. P.

Contents:

An Introduction to the Symposium.- Symposium Lecture.- Developments in X-ray Technology and Their Contribution to Structural Biology.- Some of the Synchrotron Facilities for Biological Structural Studies.- MacChess - A Macromolecular Diffraction Resource at the Cornell High Energy Synchrotron Source.- Facilities Available for Biophysics Research at the Stanford Synchrotron Radiation Laboratory.- Synchrotron Radiation Facilities for Biological Use at the Institute for Solid State Physics and Photon Factory.- Biological Structural Studies at the LURE Synchrotron Radiation Facility.- Synchrotron Beamlines at the European Molecular Biology Laboratory Outstation In Hamburg.- Research Facilities for Biology at the Synchrotron Radiation Source at Daresbury Laboratory.- Facilities at the National Synchrotron Light Source at Brookhaven National Laboratory.- Small Angle X-Ray Scattering.- Synchrotron Radiation Time-Resolved Solution X-ray Scattering: The Example of Clathrin Structure and Assembly.- Large-Scale Structural Changes in the Sarcoplasmic Reticulum ATP-ASE Are Essential for Calcium Active Transport.- Anomalous Scattering in Membrane Studies.- Synchrotron X-ray Studies of Biopolymers: Self-Assembly and Oscillations of Microtubules.- X-Ray Crystallography.- Structural Studies of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from Spinach.- Data Collection from Very Thin HLA Crystals Using Synchrotron Radiation.- Double-Stranded DNA Binding Protein Hu.- Quaternary and Tertiary Structures of Isometric RNA Viruses.- X-Ray Spectroscopy.- Iron Core Formation in Ferritins.- X-ray Absorption Spectroscopic Studies of Vanado-Enzymes Nitrogenase and Bromoperoxidase.- X-ray Absorption Spectroscopy of Pseudomonas Cepacia Phthalate Dioxygenase.- Ultraviolet and Visible Spectroscopy and Photobiology.- Fluorescence Spectral Characteristics and Fluorescence Decay Profiles of Covalent Polycyclic Aromatic Carcinogen-DNA Adducts.- Time-resolved Fluorescence in Studies of Protein Structure and Dynamics.- Vacuum Ultraviolet Photobiology with Synchrotron Radiation.- Advances in X-Ray Instrumentation.- Detectors for High Photon Rates.- X-ray Optics for Synchrotron Radiation.- Sagittal Focusing Optics.- Understanding the Performance of X-ray Mirrors.- New Methods in Use of Synchrotron Radiation for Structural Biology.- Multiwavelength Anomalous Diffraction as a Direct Phasing Vehicle in Macromolecular Crystallography.- Laue Photography from Protein Crystals.- Laue Crystallography: Application to Virus Crystals.- Preparation of Magnetically Oriented Specimens for Diffraction Experiments.- Participants.