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Practical Guide to the Histology of the Mouse
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Main description:

A Practical Guide to the Histology of the Mouse provides a full–colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year. Complete phenotypic analysis of all of these models can benefit from histologic review of the tissues.

This book is aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models. It provides practical guidance on the collection, sampling and analysis of mouse tissue samples in order to maximize the information that can be gained from these tissues. As well as illustrating the normal microscopic anatomy of the mouse, the book also describes and explains the common anatomic variations, artefacts associated with tissue collection and background lesions to help the scientist to distinguish these changes from experimentally– induced lesions.


This will be an essential bench–side companion for researchers and practitioners looking for an accessible and well–illustrated guide to mouse pathology.



  • Written by experienced pathologists and specifically tailored to the needs of scientists and histologists

  • Full colour throughout

  • Provides advice on sampling tissues, necropsy and recording data

  • Includes common anatomic variations, background lesions and artefacts which will help non–experts understand whether histologic variations seen are part of the normal background or related to their experimental manipulation


Back cover:

A Practical Guide to the Histology of the Mouse provides a full–colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year. Complete phenotypic analysis of all of these models can benefit from histologic review of the tissues.

This book is aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models. It provides practical guidance on the collection, sampling and analysis of mouse tissue samples in order to maximize the information that can be gained from these tissues. As well as illustrating the normal microscopic anatomy of the mouse, the book also describes and explains the common anatomic variations, artefacts associated with tissue collection and background lesions to help the scientist to distinguish these changes from experimentally– induced lesions.


This will be an essential bench–side companion for researchers and practitioners looking for an accessible and well–illustrated guide to mouse pathology.



  • Written by experienced pathologists and specifically tailored to the needs of scientists and histologists

  • Full colour throughout

  • Provides advice on sampling tissues, necropsy and recording data

  • Includes common anatomic variations, background lesions and artefacts which will help non–experts understand whether histologic variations seen are part of the normal background or related to their experimental manipulation


Contents:

List of contributors ix

Foreword xi


Preface xiii


About the companion website xv


Chapter 1 Necropsy of the mouse 1
Lorna Rasmussen and Elizabeth McInnes


1.1 Recording of findings 2


1.2 Bleeding technique 3


1.3 Perfusion 3


1.4 External examination 4


1.5 Weighing of organs 6


1.6 Positioning of mouse for necropsy and removing the skin 6


1.7 Opening the abdominal cavity and exposing organs 9


1.8 Removing the ribcage to expose lungs and heart 17


1.9 Removing the brain and spinal cord 20


1.10 Collecting and fixing tissue samples 22


References 22


Chapter 2 Practical approaches to reviewing and recording pathology data 25
Cheryl L. Scudamore


2.1 Sample selection and trimming patterns 26


2.2 Controls 28


2.3 Standardizing terminology 28


2.4 Microscopic terminology 30


2.5 Recording pathology data 33


2.6 Quantitative versus semiquantitative analysis 35


2.7 Semiquantitative techniques 37


2.8 Quantitative techniques 38


References 40


Chapter 3 Gastrointestinal system 43
Cheryl L. Scudamore


3.1 Background and development 43


3.2 Oral cavity 43


3.3 Salivary glands 46


3.4 Stomach and intestines 48


3.5 Liver 56


3.6 Pancreas 59


References 61


Chapter 4 Cardiovascular system 63
Cheryl L. Scudamore


4.1 Background and development 63


4.2 Sampling techniques and morphometry 63


4.3 Artefacts 65


4.4 Anatomy and histology of the heart 67


4.5 Anatomy and histology of the blood vessels 69


References 72


Chapter 5 Urinary system 75
Elizabeth McInnes


5.1 Background and development 75


5.2 Sampling techniques 76


5.3 Artefacts 76


5.4 Background lesions 77


5.5 Anatomy and histology 79


References 85


Chapter 6 Reproductive system 87
Cheryl L. Scudamore


6.1 Background and development of the male and female reproductive tract 87


6.2 Female reproductive tract 88


6.3 Male reproductive tract 97


References 105


Chapter 7 Endocrine system 109
Ian Taylor


7.1 Introduction 109


7.2 Adrenals 109


7.3 Pituitary 114


7.4 Thyroids and parathyroids 117


References 121


Chapter 8 Nervous system 123
Aude Roulois


8.1 Introduction 123


8.2 Necropsy 123


8.3 Fixation/perfusion 126


8.4 Trimming 131


8.5 Special stains and techniques 133


8.6 Microscopic examination 137


8.7 Normal histology–juvenile CNS and PNS 137


8.8 Artefacts CNS and PNS 141


8.9 Pathological changes and background pathology CNS and PNS 142


8.10 Acknowledgment 146


References 146


Useful website resources 148


Chapter 9 Lymphoid and haematopoietic system 149
Ian Taylor


9.1 Introduction 149


9.2 Lymph nodes 150


9.3 Spleen 156


9.4 MALT (mucosa–associated lymphoid tissue) 160


9.5 Thymus 161


9.6 Bone marrow 164


References 166


Chapter 10 Integument and adipose tissue 169
Cheryl L. Scudamore


10.1 Background and development 169


10.2 Sampling technique 170


10.3 Anatomy and histology 171


References 177


Chapter 11 The respiratory system 179
Elizabeth McInnes


11.1 Background and development 179


11.2 Embryology 179


11.3 Anatomy and histology of the respiratory system 180


11.4 Upper respiratory tract 180


11.5 Lower respiratory tract 189


References 193


Chapter 12 Special senses 195
Cheryl L. Scudamore


12.1 Background and development 195


12.2 Sampling technique for the eye 195


12.3 Artefacts 197


12.4 Anatomy and histology of the eye and associated glands 199


12.5 Background and development of the ear 204


12.6 Sampling technique for the ear and associated structures 205


12.7 Anatomy and histology of the ear and associated glands 206


References 209


Chapter 13 Musculoskeletal system 211
Cheryl L. Scudamore


13.1 Background and development 211


13.2 Sampling techniques 211


13.3 Anatomy and histology 212


References 218


Index 221


PRODUCT DETAILS

ISBN-13: 9781118789476
Publisher: John Wiley & Sons Ltd (Wiley–Blackwell)
Publication date: February, 2014
Pages: 352
Dimensions: 189.00 x 251.00 x 17.88

Subcategories: Anatomy

MEET THE AUTHOR

Cheryl L Scudamore is currently Head of Pathology at Mary Lyon Centre, MRC Harwell and an Honorary Professor in Experimental Pathology at the Royal Veterinary College. She has worked extensively as a pathologist in academia and industry in the UK and is known for her interest in and promotion of postgraduate training in laboratory animal pathology and histological techniques.