Phage Display in Biotechnology and Drug Discovery, Second Edition provides a comprehensive view of the impact and promise of phage display in drug discovery and biotechnology. Building on the success of its previous edition, the book discusses current theories, principles, and methods in the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. The book provides readers with an overview of the amazing breadth of the impact that phage display technology has had on the study of proteins in general as well as the development of proteins. It will be a valuable resource for those interested in using phage display and recombinant antibodies in basic research and drug discovery.
Filamentous Bacteriophage Structure and Biology Diane J. Rodi, Suneeta Mandava, and Lee Makowski Vectors and Modes of Display Valery A. Petrenko and George P. Smith Methods for the Construction of Phage-Displayed Libraries Frederic A. Fellouse and Gabor Pal Selection and Screening Strategies Mark S. Dennis Leveraging Synthetic Phage-Antibody Libraries for Panning on the Mammalian Cell Surface Jelena Tomic, Megan McLaughlin, Traver Hart, Sachdev Sidhu, and Jason Moffat Phage Libraries for Developing Antibody-Targeted Diagnostics and Vaccines Nienke E. van Houten and Jamie K. Scott Exploring Protein-Protein Interactions Using Peptide Libraries Displayed on Phage Kurt Deshayes Substrate Phage Display Shuichi Ohkubo Mapping Intracellular Protein Networks Malgorzata E. Kokoszka, Zhaozhong Han, Ece Karatan, and Brian K. Kay High-Throughput and High-Content Screening Using Peptides Robert O. Carlson, Robin Hyde-Deruyscher, and Paul T. Hamilton Engineering Protein Folding and Stability Mihriban Tuna and Derek N. Woolfson Identification of Natural Protein-Protein Interactions with cDNA Libraries Reto Crameri, Claudio Rhyner, Michael Weichel, Sabine Fluckiger, and Zoltan Konthur Mapping Protein Functional Epitopes Sara K. Avrantinis and Gregory A. Weiss Selections for Enzymatic Catalysts Julian Bertschinger, Christian Heinis, and Dario Neri Antibody Humanization and Affinity Maturation Using Phage Display Jonathan S. Marvin and Henry B. Lowman Antibody Libraries from Immunized Repertoires Jody D. Berry and Mikhail Popkov Naive Antibody Libraries from Natural Repertoires Claire L. Dobson, Ralph R. Minter, and Celia P. Hart-Shorrock Synthetic Antibody Libraries Frederic A. Fellouse and Sachdev S. Sidhu Engineering Antibody Fragments for Intracellular Applications Jianghai Liu and C. Ronald Geyer