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Handbook of RNA Biochemistry
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Main description:

An all-in-one compendium for everyone working with small or large RNAs. Contains hundreds of protocols, compelete with explanations and troubleshooting hints not found elsewhere.


Contents:

RNA SYNTHESIS Enyzmatic synthesis, ligation and modification Chemical synthesis NEW: RNA detection and quantitation STRUCTURE DETERMINATION Sequence determination NEW: Probing in vitro and in vivo Footprinting techniques Nucleotide analog interference mapping Identification of interacting proteins and nucleic acids Spectroscopic studies of RNA structure Atomic structure determination NEW: Functional assays NEW: Mass spectrometry of RNA NEW: Folding and unfolding of single RNA molecules RNA GENOMICS & BIOINFORMATICS Phylogenetic analysis Structure prediction Modeling of RNA structures Molecular dynamics simulation NEW: Searching and predicting small RNAs NEW: RNA mining and RNomics in eukaryotes NEW: Exon arrays ANALYSIS OF RNA-PROTEIN INTERACTION Isolation and purification of RNA-binding proteins Sedimentation analysis of RNP complexes Protein-RNA crosslinking Immunoaffinity purification NEW: TAP-tag affinity purification Three-hybrid analysis to identify RNA-binding proteins SELEX In vitro selection of ribozymes and aptamers In vivo SELEX In vitro selection against small targets NEW: Genomic SELEX RNA INTERFERENCE NEW: Chemical modification of siRNA NEW: siRNA design and delivery NEW: RNAi in mammalian cells (partial contents)


PRODUCT DETAILS

ISBN-13: 9783527650552
Publisher: John Wiley & Sons Ltd (Wiley-VCH Verlag GmbH)
Publication date: January, 2013
Pages: 1300

Subcategories: Biochemistry