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Epithelial Cell Culture Protocols
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Main description:

There have been significant advances in research involving the isolation and culture of epithelial cells in the past decade, and many new techniques have been developed. Monolayer cultures can be used to evaluate the nature and behavior of cells, while the use of epithelial cells in model systems has allowed a deeper understanding of cellular and molecular mechanisms and interactions. The aim of this book is to provide a comprehensive, step-by-step guide to many techniques for epithelial cell culture, combining in one volume the more commonly used protocols along with many that are more speci- ized. Epithelial Cell Culture Protocols should help those who are new to this field and want to learn the basic culture techniques, as well as those needing to use more wide ranging and specific protocols. It should be a useful resource on its own, and also complement the other volumes that have been written about cell culture in the Methods in Molecular Biology series. Epithelial Cell Culture Protocols covers a wide variety of protocols, mostly aimed at the researcher, but also a few aimed at clinicians. The est- lishment and maintenance of primary cultures derived from many different tissues and different species is covered. Particular emphasis has been placed on protocols needed to further analyze and assess epithelial cells, for example, by looking at apoptosis and integrins and by measuring membrane capa- tance and confluence. Using different co-culture techniques, it is possible also to develop models to investigate many different systems in vitro.

Back cover:

Many powerful new techniques for the isolation and culture of epithelial cells have been developed in the past decade. In Epithelial Cell Culture Protocols, a team of well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. These readily reproducible techniques describe all the commonly used protocols, as well as those that are more specialized, including several aimed at clinical investigation. The emphasis is on the analysis and assessment of epithelial cells, for example, by looking at apoptosis and integrins or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Several protocols cover the culturing of epithelial cells and their use in treating patients with burns and other skin disorders.

Wide-ranging and highly practical, Epithelial Cell Culture Protocols offers both novices and expert investigators alike a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms in general, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.


Human Lens Epithelial Cell Culture
Nobuhiro Ibaraki

Human Airway Epithelial Cell Culture
Mutsuo Yamaya, Masayoshi Hosoda, Tomoko Suzuki, Norihiro Yamada, and Hidetada Sasaki

Rat Gastric Mucosal Epithelial Cell Culture
Yoshitaka Konda and Tsutomu Chiba

Thymic Epithelial Cell Culture
Carsten Röpke

Bile Duct Epithelial Cell Culture
Alphonse E. Sirica

Liver Epithelial Cell Culture
Rolf Gebhardt

Pulmonary Epithelial Cell Culture
Ben Forbes

Prostate Epithelial Cell Isolation and Culture
David L. Hudson

A Bovine Mammary Endothelial/Epithelial Cell Culture Model of the Blood/Milk Barrier
Albert Guidry and Celia O'Brien

The Blood-CSF Barrier in Culture: Development of a Primary Culture and Transepithelial Transport Model for Choroidal Epithelial Cells
Wei Zheng and Qiuqu Zhao

An In Vitro Model of Differentiated Human Airway Epithelia: Methods for Establishing Primary Cultures
Philip H. Karp, Thomas O. Moninger, S. Pary Weber, Tamara S. Nesselhauf, Janice L. Launspach, Joseph Zabner, and Michael J. Welsh

Primary Mouse Keratinocyte Culture
Kairbaan Hodivala-Dilke

Analyzing Apoptosis in Cultured Epithelial Cells
Andrew P. Gilmore and Charles H. Streuli

Keratins as Markers of Epithelial Cells
David L. Hudson

Epithelial Cell Integrins
Joachim Rychly

Isolation, Cultivation, and Differentiation of Normal Human Epidermal Keratinocytes in Serum-Free Medium
Sebastian Zellmer and Dieter Reissig

Clinical Application of Autologous Cultured Epithelia for the Treatment of Burns and Disfigurement of Skin Surfaces
Norio Kumagai

Transplantation of Cultured Epithelial Cells
Minoru Ueda, Yukio Sumi, Yoshitaka Hibino, and Ken-Ichiro Hata

An Outgrowth Culture System of Normal Human Epithelium: An In Vitro Modelto Study the Coordination of Cellular Migration and Proliferation
Tohru Masui

Application of Epithelial Cell Culture in Drug Transport in the Respiratory Tract
Jie Shen and Vincent H. L. Lee

Applications of Epithelial Cell Culture in Studies of Drug Transport
Staffan Tavelin, Johan Gråsjö, Jan Taipalensuu, Göran Ocklind, and Per Artursson

X-Ray Microanalysis of Epithelial Cells in Culture
Godfried M. Roomans

ENU Mutagenesis of Rat Mammary Epithelial Cells
George Stoica

ENU-Induced Ovarian Cancer
George Stoica

Measurement of Membrane Capacitance in Epithelial Monolayers
Carol A. Bertrand and Ulrich Hopfer

Assessing Epithelial Cell Confluence by Spectroscopy
Simon A. Lewis

Co-Cultivation of Liver Epithelial Cells with Hepatocytes
Rolf Gebhardt

Co-Culture and Crosstalk between Endothelial Cells and Vascular Smooth Muscle Cells Mediated by Intracellular Calcium
Wee Soo Shin, Chieko Hemmi, and Teruhiko Toyo-oka

Establishing Epithelial-Immune Cell Co-Cultures: Effects on Epithelial Ion Transport and Permeability
Derek M. McKay and Mary H. Perdue

Explant Cultures of Embryonic Epithelium: Analysis of Mesenchymal Signals
Carin Sahlberg, Tuija Mustonen, and Irma Thesleff

Bacterial Interactions with Host Epithelium In Vitro
Nicola Jones, Mary H. Perdue, Philip M. Sherman, and Derek M. McKay


ISBN-13: 9781617372414
Publisher: Springer (Humana Press)
Publication date: October, 2010
Pages: 420
Weight: 708g
Availability: POD
Subcategories: Respiratory Medicine
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