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Published November, 2009
By Nejat Duzgunes
Publisher: Elsevier (Academic Press)
Series: Methods in Enzymology
Liposomes are cellular structures made up of lipid molecules, which are water insoluble organic molecules and the basis of biological membranes. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Part F is a continuation of previous MIE Liposome volumes A through E. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences

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Published November, 2009
By Nejat Duzgunes
Publisher: Elsevier (Academic Press)
Series: Methods in Enzymology
Liposomes are cellular structures made up of lipid molecules, which are water insoluble organic molecules and the basis of biological membranes. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Part F is a continuation of previous MIE Liposome volumes A through E. * One of the most highly respected publications in the field of biochemistry since 1955 * Frequently consulted and praised by researchers and reviewers alike * Truly an essential publication for anyone in any field of the life sciences

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£117.00
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Published November, 2009
By Richard R Burgess and Murray P. Deutscher
Publisher: Elsevier (Academic Press)
Series: Methods in Enzymology
The 2e of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "e;Guide to"e; gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniquesProvides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

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£117.00
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Published September, 2009
By Bastien D. Gomperts, Ijsbrand M. Kramer and Peter E.R. Tatham
Publisher: Elsevier (Academic Press)
Signal Transduction, 2e, is a thorough, well-illustrated study in cellular signaling processes. Beginning with the basics, this book shows how cells respond to external cues, hormones, growth factors, cytokines, cell surfaces, etc., and further instructs how these inputs are integrated. Instruction continues with up-to-date, inclusive coverage of intracellular calcium, nuclear receptors, tyrosine protein kinases and adaptive immunity, and targeting transduction pathways for research and medical intervention. Signal Transduction, 2e, serves as an invaluable resource for advanced undergraduates, graduate researchers, and established scientists working in cell biology, pharmacology, immunology, and related fields.Up-to-date, inclusive coverage of targeting transduction pathways for research and medical intervention In-depth coverage of nuclear receptors, including steps in isolation of steroid hormones and the discovery of intracellular hormone receptors; tyrosine protein kinases and adaptive immunity; and intracellular calciumExtensive conceptual color artwork to assist with comprehension of key topicsInstrumental margin notes highlight milestones in signaling mechanisms

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£56.99
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Published October, 2009
By Geoffrey Cordell
Publisher: Elsevier (Academic Press Inc)
Presents coverage of particular classes or sources of alkaloids. This title provides coverage of the chemical and biological aspects of important natural products.

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£145.00
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Published July, 2009
By Tom W. Muir and John N. Abelson
Publisher: Elsevier (Academic Press)
Series: Methods in Enzymology
By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tubePresents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural moleculesProvides a "e;one-stop shop"e; for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods

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£117.00
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Published July, 2009
By Antony Bacic, Geoffrey B. Fincher and Bruce A. Stone
Publisher: Elsevier (Academic Press)
Chemistry, Biochemistry, and Biology of 1-3 Beta Glucans and Related Polysaccharides presents a comprehensive, systematic and authoritative survey of information about a family of chemically related, but functionally diverse, naturally occurring polysaccharides--the (1-3)-glucans. International contributors describe the chemical and physicochemical properties of these glucans and their derivatives and the molecular biological and structural aspects of the enzymes involved in their formation and breakdown. A detailed analysis of their physiological roles in the various biological situations in which they are found will be provided. Additionally, evolutionary relationships among the family of these glucans will be described.Topics of medical relevance include detailing the glucans' interactions with the immune system and research for cancer therapy applicationsWeb resource links allow scientists to explore additional beta glucan researchSeparate indexes divided into Species and Subject for enhanced searchability

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£81.00
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Published April, 2009
By David A. Hopwood
Publisher: Elsevier (Academic Press)
Series: Methods in Enzymology
Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine.* Gathers tried and tested methods and techniques from top players in the field.* Provides an extremely useful reference for the experienced research scientist.* Covers biosynthesis of Polyketides, Tarpenoids, Aminocoumarins and Crabohydrates

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£117.00
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Published April, 2009
By David A. Hopwood
Publisher: Elsevier (Academic Press)
Series: Methods in Enzymology
Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine.* Gathers tried and tested methods and techniques from top players in the field.* In depth coverage of ribosomally-synthesised and Non-ribosomally-synthesised peptides.* Provides an extremely useful reference for the experienced research scientist.

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£117.00
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Published January, 2009
By Lynne E. Maquat and Megerditch Kiledjian
Publisher: Elsevier (Academic Press)
Series: Methods in Enzymology
Specific complexes of protein and RNA carry out many essential biological functions, including RNA processing, RNA turnover, RNA folding, as well as the translation of genetic information from mRNA into protein sequences. Messenger RNA (mRNA) decay is now emerging as an important control point and a major contributor to gene expression. Continuing identification of the protein factors and cofactors, and mRNA instability elements responsible for mRNA decay allow researchers to build a comprehensive picture of the highly orchestrated processes involved in mRNA decay and its regulation.Covers the nonsense-mediated mRNA decay (NMD) or mRNA surveillance pathwayExpert researchers introduce the most advanced technologies and techniques to identify mRNA processing, transport, localization and turnover, which are central to the process of gene expressionOffers step-by-step lab instructions, including necessary equipment and reagents

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£125.00
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