Main description:

This volume covers the latest methods used in clinical neurochemistry laboratories for both clinical practice and research. Chapters in this book discuss topics such as techniques for cerebrospinal fluid (CSF) collection, pre-analytical processing, and basic CSF analysis; an examination of biomarkers including ELISA and automated immunochemical assays for amyloid and tau markers for Alzheimer's disease; the analysis of neurofilaments by digital ELISA; and an example of successful novel immunoassay development. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and thorough, Cerebrospinal Fluid Biomarkers is a valuable resource for clinicians and researchers to use in CSF labs and CSF courses.

Contents:

Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1. CSF Cell: Cell Count, Cytomorphology, Cytology, and Immunophenotyping
Sigurd D. Sussmuth and Hayrettin Tumani

2. Nephelometry and Turbidimetry: Methods to Quantify Albumin and Immunoglobins Concentrations in Clinical Neurochemistry
Piotr Lewczuk

3. Oligoclonal Bands: Isoelectric Focusing and Immunoblotting, and Determination of Free Light Chains in the Cerebrospinal Fluid
Harald Hegen and Florian Deisenhammer

4. Abeta CSF LC-MS
Josef Pannee, Kaj Blennow, and Henrik Zetterberg

5. Immunoassay and Mass Spectrometry Methods for Tau Protein Quantification in the Cerebrospinal Fluid
Christophe Hirtz, Jerome Vialaret, Constance Delaby, Aleksandra Maleska Maceski, Cyndi Catteau, Nelly Ginestet, Audrey Gabelle, and Sylvain Lehmann

6. CSF RT-QuIC and the Diagnosis of Creutzfeldt-Jakob Disease
Alison J. E. Green and Neil I. McKenzie

7. Lumbar Puncture: Consensus Guidelines
Pim Fijneman and Sebastiaan Engelborghs

8. Pre-Analytical Processing and Biobanking Protocol for CSF Samples
Charlotte Teunissen and Eline Willemse

9. Communicating Complex Results of Cerebrospinal Fluid Analysis
Axel Regeniter and Werner H. Siede

10. Method and Clinical Validation of Biomarkers for Neurodegenerative Diseases
Ulf Andreasson and Henrik Zetterberg

11. Sample Preparation for Proteomic Analysis of Cerebrospinal Fluid
Johan Gobom

12. Brain Biomarkers: Follow Up of RNA Expression Discovery Approach - CSF Assays for Neurogranin, SNAP-25, and VILIP-1
Elizabeth M. Herries, Nancy Brada, Courtney L. Sutphen, Anne M. Fagan, and Jack H. Ladenson

13. Quantification of the Neurofilament Light Chain Protein by Single Molecule Array (Simoa) Array
Christian Barro, Sarah Storz, Svenya Groebke, Zuzanna Michalak, and Jens Kuhle

Subject Index List...