Main description:

This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.

Contents:

1. Evaluation of prototype CE-MS interfaces

Sabrina Ferre, Julien Boccard, Serge Rudaz, and Victor Gonzalez-Ruiz

2. Capillary Electrophoresis-Mass Spectrometry (CE-MS) by Sheath-Flow Nanospray interface and its use in biopharmaceutical applications

Mei Han, Richard Smith, and Dan A. Rock

3. Protocol for etching bare-fused silica capillaries for sheathless capillary electrophoresis-mass spectrometry coupling

Clarisse Gosset-Erard, Jeremie Giorgetti, Michael Biacchi, Frederic Aubriet, Emmanuelle Leize-Wagner, Patrick Chaimbault, and Yannis-Nicolas Francois

4. Microfabricated Liquid Junction Capillary Electrophoresis-Mass Spectrometry Interface

Jana Krenkova and Frantisek Foret

5. Successive Multiple Ionic-Polymer Layer Coatings for intact protein analysis by Capillary Zone Electrophoresis - Mass Spectrometry - Application to Hemoglobin Analysis

Liesa Salzer, Alexander Stolz, Laura Dhellemmes, Alisa Hoechsmann, Laurent Leclercq, Herve Cottet, and Christian Neususs

6. On-line Immobilized Enzyme Microreactor Capillary Zone Electrophoresis-Mass Spectrometry for Peptide Mapping

Roger Pero-Gascon, Laura Pont, Estela Gimenez, Victoria Sanz-Nebot and Fernando Benavente

7. Bottom up analysis of proteins by peptide mass fingerprinting with tCITP-CZE-ESI-TOF MS after tryptic digest

Marius Segl and Hanno Stutz

8. Top-down proteomics by capillary zone electrophoresis-tandem mass spectrometry for large-scale characterization of proteoforms in complex samples

Elijah N. McCool, Rachele A. Lubeckyj, Daoyang Chen, and Liangliang Sun

9. Assessment of macro- and microheterogeneity of monoclonal antibodies using Capillary Zone Electrophoresis hyphenated with Mass Spectrometry

Christoph Gstoettner, Rob Haselberg, Manfred Wuhrer, Govert W. Somsen, Elena Dominguez-Vega

10. High Sensitivity Glycoproteomic Analysis of Biological Samples by CZE-ESI-MS

Wei Wang and Guinevere S.M. Lageveen-Kammeijer

11. Capillary Zone Electrophoresis-Electrospray Ionization Tandem Mass Spectrometry for Total Analysis of Chondroitin/Dermatan Sulfate Oligosaccharides

Alina D. Zamfir

12. In-Source Fragmentation for Identification of Compounds by CE-ESI-TOF in Human Plasma. L-Proline as case study

Maricruz Mamani-Huanca, Ana Gradillas, Angeles Lopez-Gonzalvez, and Coral Barbas

13. Assessing the energy status of low numbers of mammalian cells by capillary electrophoresis-mass spectrometry

Wei Zhang, Rawi Ramautar

14. Applications of Capillary Electrophoresis-Mass Spectrometry to Chiral Analysis

Elena Sanchez-Lopez and Maria Luisa Marina

15. Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extraction coupled to Capillary Electrophoresis-Tandem Mass Spectrometry

Francisco J. Lara and A.M. Garcia-Campana

16. CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis

Darya Mozhayeva and Carsten Engelhard