Main description:

This detailed volume gathers a comprehensive collection of methods, protocols, and procedures used for the identification, characterization, and selection of cancer stem cells. These include surface marker expression, side population, spheres formation, ALDH activity, and numerous others. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert tips and troubleshooting advice to avoid potential pitfalls.
Comprehensive and practical, Cancer Stem Cells: Methods and Protocols provides researchers with the techniques they will need in order to pursue vital research into this complex field of study.

Contents:

1. Introduction to Cancer Stem Cells: Past, Present, and Future

David Bakhshinyan, Ashley A. Adile, Maleeha A. Qazi, Mohini Singh, Michelle M. Kameda-Smith, Nick Yelle, Chirayu Chokshi, Chitra Venugopal, and Shiela K. Singh

2. Surface Markers for the Identification of Cancer Stem Cells

Vinod Gopalan, Farhadul Islam, and Alfred King-yin Lam

3. The Role of CD44 and Cancer Stem Cells

Liang Wang, Xiangsheng Zuo, Keping Xie, and Daoyan Wei

4. Evaluation and Isolation of Cancer Stem Cells Using ALDH Activity Assay

Luigi Mele, Davide Liccardo, and Virginia Tirino

5. Isolation of Cancer Stem Cells by Side Population Method

Masayuki Shimoda, Masahide Ota, and Yasunori Okada

6. Self-Renewal and CSCs In Vitro Enrichment: Growth as Floating Spheres

Pooja Mehta, Caymen Novak, Shreya Raghavan, Maria Ward, and Geeta Mehta

7. In Vitro Tumorigenic Assay: The Tumor Spheres Assay

Hui Wang, Anna Paczulla, Martina Konantz, and Claudia Lengerke

8. In Vitro Tumorigenic Assay: Colony Forming Assay for Cancer Stem Cells

Vijayalakshmi Rajendran and Mayur Vilas Jain

9. Xenograft as In Vivo Experimental Model

Manuela Porru, Luca Pompili, Carla Caruso, and Carlo Leonetti

10. How to Assess Drug Resistance in Cancer Stem Cells

Maria Laura De Angelis, Ruggero De Maria, and Marta Baiocchi

11. Tumor Tissue Analogs for Assessment of Radioresistance in Cancer Stem Cells

Meenakshi Upreti

12. Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)

Anna Di Biase, Amanda K. Miles, and Tarik Regad

13. Identification and Isolation of Cancer Stem Cells Using NANOG-EGFP Reporter System

Magdalena E. Buczek, Stephen P. Reeder, and Tarik Regad

14. Determination of miRNAs from Cancer Stem Cells Using a Low Density Array Platform

Hiromichi Kawasaki, Angela Lombardi, and Michele Caraglia

15. Assessing DNA Methylation in Cancer Stem Cells

Sudipto Das, Bruce Moran, and Antoinette S. Perry

16. Histones Acetylation and Cancer Stem Cells (CSCs)

Vivian Petersen Wagner, Manoela Domingues Martins, and Rogerio Moraes Castilho

17. Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods

Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Giovanna Roncador, Federica Zito Marino, and Renato Franco

18. Circulating Tumor Cells

Sebastian A. Garcia, Jurgen Weitz, and Sebastian Schoelch