Main description:

This detailed volume focuses on the development of mycosis vaccines via the most common etiological agents of mycoses, such as Aspergillus and Candida species, followed by Cryptococcus, dimorphic fungi, and members of Mucoraceae family of fungi. The book is sectioned into five parts that describe experimental approaches for vaccine candidate discovery, improved vaccine design and delivery, assessment of critical endpoints, analysis of mechanisms of mycosis vaccine protection, and key methods for clinical translation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting known pitfalls.
Practical and authoritative, Vaccines for Invasive Fungal Infections: Methods and Protocols provides a standard reference for vaccine researchers, including those who aspire to strengthen their knowledge or desire to acquire a particular technique.

Contents:

Part I: Discovery and Testing of Vaccine Candidates

1. Mouse Immunization with Radioattenuated Yeast Cells of Paracoccidioides brasiliensis

Estefania Mara do Nascimento Martins and Antero Silva Ribeiro de Andrade

2. Heat-Killed Yeast as a Pan-Fungal Vaccine

Marife Martinez, Karl V. Clemons, and David A. Stevens

3. Immunoinformatics as a Tool for New Antifungal Vaccines

Rupanjali Chaudhuri and Srinivasan Ramachandran

4. Rational Design of T Lymphocyte Epitope-Based Vaccines Against Coccidioides Infection

Brady J. Hurtgen and Chiung-Yu Hung

5. Identification of Fungal T Cell Epitopes by Mass Spectrometry-Based Proteomics

Bernd Roschitzki and Salome LeibundGut-Landmann

6. Intranasal Antifungal Vaccination Using DNA-Transfected Dendritic Cells

Shanjana Awasthi

7. DNAhsp65 Vaccine as Therapy Against Paracoccidioidomycosis

Alice M. Ribeiro, Andre C. Amaral, Maria Sueli S. Felipe, and Anamelia L. Bocca

8. Idiotypic Antifungal Vaccination: Immunoprotection by Antiidiotypic Antibiotic Antibodies

Luciano Polonelli, Walter Magliani, and Stefania Conti

9. Peptide Vaccine Against Paracoccidioidomycosis

Carlos P. Taborda and Luiz R. Travassos

10. Methodology for Anti-Cryptococcal Vaccine Development

Ashok K. Chaturvedi and Floyd L. Wormley Jr.

Part II: Design and Delivery

11. Beta-Glucan Particles as Vaccine Adjuvant Carriers

Zeynep Mirza, Ernesto R. Soto, Fusun Dikengil, Stuart M. Levitz, and Gary R. Ostroff

12. Th1-Inducing Agents in Prophylaxis and Therapy for Paracoccidioidomycosis

Thiago Aparecido da Silva, Fabricio Freitas Fernandes, Taise Natali Landgraf, Ademilson Panunto-Castelo, and Maria Cristina Roque-Barreira

13. Nanoparticle-Based Mycosis Vaccine

Swaleha Zubair, Asim Azhar, Nazoora Khan, Ejaj Ahmad, Mohd Ajmal, and Owais Mohammad

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14. Yeast Expressing Gp43 Protein as a Vaccine Against Paracoccidioides brasiliensis Infection

Aline Ferreira Oliveira and Paulo S.R. Coelho

15. Vaccination with Phage-Displayed Antigenic Epitope

Yicun Wang and Li Wang

16. Preparation of an Oral Vaccine by Proteome Analysis and Molecular Display Technology

Seiji Shibasaki and Mitsuyoshi Ueda

Part III: Endpoint Assessment

17. Precise and Efficient In-Frame Integration of an Exogenous GFP Tag in Aspergillus fumigatus by a CRISPR System

Chi Zhang and Ling Lu

18. Endpoint Assessment in Rabbit Models of Invasive Pulmonary Aspergillosis and Mucormycosis

Vidmantas Petraitis, Ruta Petraitiene, William Hope, and Thomas J. Walsh

Part IV: Mechanism of Vaccine Protection

19. CD4+ T Cells Mediate Aspergillosis Vaccine Protection

Diana Diaz-Arevalo and Markus Kalkum

20. T-Cell-Mediated Cross-Protective Immunity

Nina Khanna and Claudia Stuehler

21. Assessment of Post-Vaccination Phagocytic Activation Using Candida albicans Killing Assays

Wenqing Li and Yan Hu

22. Immunization with Antigen-Pulsed Dendritic Cells Against Highly Virulent Cryptococcus gattii Infection: Analysis of Cytokine-Producing T Cells

Keigo Ueno, Makoto Urai, Shogo Takatsuka, Masahiro Abe, Yoshitsugu Miyazaki, and Yuki Kinjo

Part V: Translation to Clinical Use

23. Testing Antifungal Vaccines in an Animal Model of Invasive Candidiasis and in Human Mucosal Candidiasis

Esther Segal