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The Renin-Angiotensin-Aldosterone System
Methods and Protocols
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Main description:

This volume discusses protocols that aid in measuring different components in the renin-angiotensin-aldosterone system (RAAS). The book also looks at the methods used to assess angiotensin peptides and discerning the influence of RAAS components on different mammalian diseases. The chapters cover topics such as the use of fluorescent substrate to measure ACE2 activity in the mouse abdominal aorta, blood pressure monitoring using the radio telemetry method, and the analysis of angiotensin metabolism in the kidney using mass spectrometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and comprehensive, The Renin-Angiotensin-Aldosterone Systems: Methods and Protocols is a valuable resource that provides scientists and researchers with the best approaches to examine RAAS.


Contents:

Preface...
Table of Contents...
Contributing Authors...
1. A Brief Introduction into the Renin-Angiotensin-Aldosterone System: New and Old Techniques
Sean E. Thatcher

2. A Color Segmentation Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining
Congqing Wu, Alan Daugherty, and Hong Lu

3. Assessment of Protein Carbonylation and Protein Tyrosine Phosphatase (PTP) Oxidation in Vascular Smooth Muscle Cells (VSMCs) using Immunoblotting Approaches
Sofia Tsiropoulou and Rhian M. Touyz

4. Methods for Studying the Role of RAAS in the Modulation of Vascular Repair-Relevant Functions of Stem/Progenitor Cells
Yagna P.R. Jarajapu

5. Use of a Fluorescent Substrate to Measure ACE2 Activity in the Mouse Abdominal Aorta
Yu Wang, Lisa A. Cassis, and Sean E. Thatcher

6. Measuring Blood Pressure using a Non-Invasive Tail Cuff Method in Mice
Yu Wang, Sean E. Thatcher, and Lisa A. Cassis

7. Blood Pressure Monitoring using Radio Telemetry Method in Mice
Yu Wang, Sean E. Thatcher, and Lisa A. Cassis

8. Characterization and Functional Phenotyping of Renal Immune Cells via Flow Cytometry
Nathan P. Rudemiller and Steven D. Crowley

9. Assessment of the Renin-Angiotensin System in Cellular Organelle: New Arenas for Study in the Mitochondria
Bryan A. Wilson and Mark C. Chappell

10. Comprehensive Assessments of Energy Balance in Mice
Justin L. Grobe

11. In Vitro Assays to Determine Smooth Muscle Cell Hypertrophy, Protein Content, and Fibrosis
Katherine J. Elliott and Satoru Eguchi

12. A New Mouse Model for Introduction of Aortic Aneurysm by Implantation of Deoxycorticosterone Acetate Pellets or Aldosterone Infusion in the Presence of High Salt
Shu Liu, Ming C. Gong, and Zhenheng Guo

13. Fluorescence-Based Binding Assay for Screening Ligands of Angiotensin Receptors
Maiia E. Bragina, Nikolaos Stergiopulos, and Rodrigo A. Fraga-Silva

14. A Primer to Angiotensin Peptide Isolation, Stability, and Analysis by Nano-Liquid Chromatography with Mass Detection
Mariola Olkowicz, Stefan Chlopicki, and Ryszard T. Smolenski

15. Analysis of Angiotensin Metabolism in the Kidney using Mass Spectrometry
Nadja Grobe and Khalid M. Elased


PRODUCT DETAILS

ISBN-13: 9781493970285
Publisher: Springer (Humana Press Inc.)
Publication date: May, 2017
Pages: 200
Weight: 5562g
Availability: Available
Subcategories: Biochemistry, Physiology

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