Main description:

This third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems. The chapters in this book discuss genetic, proteomic, genomic, biochemical, and molecular approaches that provide insight into the complex protein interaction networks active in the telomere chromatin. This book also details information regarding telomere dynamics in response to stress or stimuli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Introduction to Telomeres and Telomerase
Zhou Songyang

2. Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9
Jun Xu, Zhou Songyang, Dan Liu, and Hyeung Kim

3. Telomere Length Analysis by Quantitative Fluorescent In Situ Hybridization (Q-FISH)
Isabelle Ourliac-Garnier and Arturo Londono-Vallejo

4. Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)
Isabelle Ourliac-Garnier and Arturo Londono-Vallejo

5. Telomere G-Rich Overhang Length Measurement: DSN Method
Yong Zhao, Jerry Shay, and Woodring E. Wright

6. Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA
Hidetoshi Tahara

7. Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In
Yong Zhao, Jerry W. Shay, and Woodring E. Wright

8. Analysis of Yeast Telomerase by Primer Extension Assays
Min Hsu and Neal F. Lue

9. Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP)
Shuai Jiang, Mengfan Tang, Huawei Xin, and Junjiu Huang

10. Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH (TM))
Miles J. McKenna, Erin Robinson, Edwin H. Goodwin, Michael N. Cornforth, and Susan M. Bailey

11. Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques
Eladio Abreu, Rebecca M. Terns, and Michael P. Terns

12. Cytogenetic Analysis of Telomere Dysfunction
Rekha Rai, Asha S. Multani, and Sandy Chang

13. Probing the Telomere Damage Response
Rekha Rai and Sandy Chang

14. Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins
Rong Tan and Li Lan

15. Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein-Protein Interactions
Wenbin Ma, Ok-hee Lee, Hyeung Kim, and Zhou Songyang

16. In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits
Jing Huang, Christopher J. Bley, Dustin P. Rand, Julian J.L. Chen, and Ming Lei

17. Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds
Clement Lin and Danzhou Yang

18. Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization
Zepeng Zhang, Qian Hu, and Yong Zhao

19. Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP)
Feng Liu, Xuyang Feng, and Wenbin Ma