Main description:

This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

Contents:

Preface... Table of Contents... Contributing Authors...

1) Super-Resolution Microscopy Techniques and their Potential for Applications in Radiation BiophysicsJan Philipp Eberle, Alexander Rapp, Matthias Krufczik, Marion Eryilmaz, Manuel Gunkel, Holger Erfle, and Michael Hausmann2) Managing the Introduction of Super-Resolution Microscopy into a Core FacilityJeffrey A. Kamykowski and Brian Storrie3) Live-Cell STED Imaging with the HyPer2 BiosensorNatalia M. Mishina and Vsevolod V. Belousov4) Diffraction-Unlimited Fluorescence Imaging with an EasySTED Retrofitted Confocal MicroscopeAndre Klauss and Carsten Hille5) Two-Photon STED Microscopy for Nanoscale Imaging of Neural Morphology In VivoMirelle J.T. ter Veer, Thomas Pfeiffer, and U. Valentin Nagerl6) STED Imaging of Golgi Dynamics with Cer-SiR: A Two-Component, Photostable, High-Density Lipid Probe for Live CellsRoman S. Erdmann, Derek Toomre, and Alanna Schepartz7) Four Channel Super-Resolution Imaging by 3-D Structured IlluminationUlrike Engel8) Correlative SIM-STORM MicroscopyO. Burri, T. Laroche, R. Guiet, and A. Seitz9) Correlative Super-Resolution Fluorescence Imaging and Atomic Force Microscopy for the Characterization of Biological SamplesPatricia Bondia, Santiago Casado, and Cristina Flors10) Quantitative Single-Molecule Localization Microscopy (qSMLM) of Membrane Proteins Based on Kinetic Analysis of Fluorophore Blinking CyclesFranziska Fricke, Joel Beaudouin, Sebastian Malkusch, Roland Eils, and Mike Heilemann11) Two-Color Single-Molecule Tracking in Live CellsSiegfried Hanselmann and Dirk-Peter Herten12) Fully Automated Targeted Confocal and Single Molecule Localization MicroscopyJan Philipp Eberle , Walter Muranyi , Hans-Georg Krausslich , Holger Erfle, and Manuel Gunkel13) Brain Slice Staining and Preparation for Three-Dimensional Super-Resolution MicroscopyChristopher L. German, Manasa V. Gudheti, Annette E. Fleckenstein, and Erik M. Jorgensen14) Correlative In-Resin Super-Resolution Fluorescence and Electron Microscopy of Cultured CellsErrin Johnson and Rainer Kaufmann15) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and their use in Cellular Imaging ExperimentsJonathan B. Grimm, Timothy A. Brown, Brian P. English, Timothee Lionnet, and Luke D. Lavis16) Measuring Nanometer Distances between Fluorescent Labels Step-by-StepSusanna Maria Fruh and Ingmar Schoen17) Correlative Single Molecule Localization Microscopy and Confocal MicroscopyChristian Soeller, Yufeng Hou, Isuru D. Jayasinghe, David Baddeley, and David Crossman18) Correlative Fluorescence Super-Resolution Localization Microscopy and Platinum Replica EM on Unroofed CellsKem A. Sochacki and Justin W. Taraska19) In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINTBrian J. Beliveau, Alistair N. Boettiger, Guy Nir, Bogdan Bintu, Peng Yin, Xiaowei Zhuang, and C.-ting Wu20) Super-Resolution High Content Screening and AnalysisK. Soliman