Main description:

This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Contents:

1. Reverse Genetics for Mammalian Orthoreovirus

Johnasha D. Stuart, Matthew B. Phillips, and Karl W. Boehme

2. Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus

Udeni B. R. Balasuriya and Jianqiang Zhang

3. Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus

Mingyuan Han, Hanzhong Ke, Yijun Du, Qingzhan Zhang, and Dongwan Yoo

4. Reverse Genetics of Zika Virus

Chao Shan, Xuping Xie, and Pei-Yong Shi

5. Efficient Reverse Genetic Systems for Rapid Genetic Manipulation of Emergent and

Pre-Emergent Infectious Coronaviruses

Adam S. Cockrell, Anne Beall, Boyd Yount, and Ralph Baric

6. Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus

Erica Bickerton, Sarah M. Keep, and Paul Britton

7. Rescue of Sendai Virus from Cloned cDNA

Shringkhala Bajimaya, Tsuyoshi Hayashi, and Toru Takimoto

8. BAC-based Recovery of Recombinant Respiratory Syncytial Virus (RSV)

Christopher C. Stobart , Anne L. Hotard, Jia Meng, and Martin L. Moore

9. Recovery of a Paramyxovirus, the Human Metapneumovirus, from Cloned cDNA

B.G. van den Hoogen and R.A.M Fouchier

10. Reverse Genetics of Newcastle Disease Virus

Stivalis Cardenas-Garcia and Claudio L. Afonso

11. Reverse Genetics Systems for Filoviruses

Thomas Hoenen and Heinz Feldmann

12. Rapid Reverse Genetics Systems for Rhabdoviruses- From Forward to Reverse and Back Again

Tobias Nolden and Stefan Finke

13. Lassa Virus Reverse Genetics

Luis Martinez-Sobrido, Slobodan Paessler, and Juan Carlos de la Torre

14. Reverse Genetics of Influenza B Viruses

Aitor Nogales, Daniel R. Perez, Jefferson Santos, Courtney Finch, and Luis Martinez-Sobrido

15. Rescue of Infectious Salmon Anemia Virus (ISAV) from Cloned cDNA

Daniela Toro-Ascuy and Marcelo Cortez-San Martin

16. Plasmid-based Reverse Genetics of Influenza a Virus

Daniel R. Perez, Matthew Angel, Ana Silvia Gonzalez-Reiche, Jefferson Santos, Adebimpe Obadan, and Luis Martinez Sobrido