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Protein NMR
Methods and Protocols
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Main description:

This volume covers state-of-the-art applications of solid-state and solution nuclear magnetic resonance( NMR) spectroscopy to study protein structure, dynamics and interactions. Chapters detail various aspects of data acquisition and processing, determination of the structure, multi-timescale dynamics of entities ranging from individual proteins to large macromolecular complexes to intact viral assemblies. The final two chapters will highlight the promise of NMR beyond field strengths of 1 GHz to study the structure, dynamics and interactions of a larger class of proteins and protein complexes of extraordinary biological interest. Written in the highly successful Methods in Molecular Biology series format, chapters provide detailed laboratory protocols and troubleshooting tips that would be of great practical help to NMR spectroscopists with different levels of expertise.

Authoritative and cutting-edge, Protein NMR: Methods and Protocol aims to ensure successful results in the further study of this vital field.


Contents:

1. NMR of Macromolecular Assemblies and Machines at 1 GHz and Beyond: New Transformative Opportunities for Molecular Structural Biology

Caitlin M. Quinn, Mingzhang Wang, and Tatyana Polenova

2. Experimental Aspects of Polarization Optimized Experiments (POE) for Magic Angle Spinning Solid-state NMR of Microcrystalline and Membrane-Bound Proteins

T. Gopinath and Gianluigi Veglia

3. Afterglow Solid-State NMR Spectroscopy

Gili Abramov and Nathaniel J. Traaseth

4. Filamentous Bacteriophage Viruses: Preparation, Magic-angle Spinning Solid-state NMR Experiments and Structure Determination

Omry Morag, Nikolaos G. Sgourakis, Gili Abramov, and Amir Goldbourt

5. Spherical Nanoparticle Supported Lipid Bilayers: A Tool for Modeling Protein
Interactions with Curved Membranes

Erin R. Tyndall and Fang Tian

6. Rapid Prediction of Multi-dimensional NMR Data Sets using FANDAS

Siddarth Narasimhan, Deni Mance, Cecilia Pinto, Markus Weingarth, Alexandre M. J. J. Bonvin, and Marc Baldus

7. Strategies for Efficient Sample Preparation for Dynamic Nuclear Polar ization Solid State NMR of Biological Macromolecules

Boris Itin and Ivan V. Sergeyev

8. In-vitro Dissolution Dynamic Nuclear Polarization for Sensitivity Enhancement of NMR with Biological Molecules

Yaewon Kim, Yunyi Wang, Hsueh-Ying Chen, and Christian Hilty

9. Determination of Protein ps-ns Motions by High-Resolution Relaxometry

Samuel F. Cousin, Pavel Kaderavek, Nicolas Bolik-Coulon, and Fabien Ferrage

10. Characterizing Protein Dynamics with NMR R1r Relaxation Experiments

Francesca Massi and Jeffrey W. Peng

11. CPMG Experiments for Protein Minor Conformer Structure Determination

Anusha B Gopalan, D Flemming Hansen, and Pramodh Vallurupalli

12. Probing the Atomic Structure of Transient Protein Contacts by Paramagnetic Relaxation Enhancement Solution NMR

Vincenzo Venditti and Nicolas L. Fawzi

13. From Raw Data to Protein Backbone Chemical Shifts Using NMRFx Processing and NMRViewJ Analysis

Bruce A. Johnson

14. Protein Structure Elucidation from NMR Data with the Program Xplor-NIH

Guillermo A. Bermejo and Charles D. Schwieters

15. Practical Nonuniform Sampling and Non-Fourier Spectral Reconstruction for Multidimensional NMR
Mark W. Maciejewski, Adam D. Schuyler, and Jeffrey C. Hoch

16. Covariance NMR Processing and Analysis for Protein Assignment

Bradley J. Harden and Dominique P. Frueh

17. Structures of Dynamic Protein Complexes: Hybrid Techniques to Study MAP Kinase Complexes and the ESCRT System

Wolfgang Peti, Rebecca Page, Evzen Boura, and Bartosz Rozycki

18. Implementation of the NMR CHEmical Shift Covariance Analysis (CHESCA): A Chemical Biologist's Approach to Allostery

Stephen Boulton, Rajeevan Selvaratnam, Rashik Ahmed, and Giuseppe Melacini

19. High-efficiency Expression of Yeast-derived G Protein-coupled Receptors and 19F Labeling for Dynamical Studies
Libin Ye, Alexander P. Orazietti, Aditya Pandey, and R. Scott Prosser

20. Quantitative Determination of Interacting Protein Surfaces in Prokaryotes and Eukaryotes by Using In-cell NMR Spectroscopy

David S. Burz, Christopher M. DeMott, Asma Aldousary, Stephen Danserea, and Alexander Shekhtman


PRODUCT DETAILS

ISBN-13: 9781493973859
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2017
Pages: 400
Weight: 652g
Availability: Available
Subcategories: Radiology

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