Main description:

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.

Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications

Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations

Covers steady-state phenomena, time-resolved phenomena, and advanced methods

Spans biophysical, biochemical, and biomedical applications

Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Contents:

Preface. Theory of Light and Light Interaction with Matter. Experimental Basics. Steady State Experiments: Trasmission/Absorption. Fluorescence: Steady-State Phenomena. Steady-State Fluorescence: Applications. Steady-State Fluorescence Polarization: Anisotropy. Fluorescence: Time-Resolved Phenomena. Advanced Experiments.