Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence.
Steady-State Phenomena Absorption and Scattering Oriented Systems and Electronic Transitions Conditions for Fluorescence Spectra Measurements Emission and Excitation Spectra of Various Fluorophores Solvent Effects: Dipole Moments of Fluorophores Fluorescence Polarization Biochemical Applications of Fluorescence Forster Resonance Energy Transfer (FRET) Time-Resolved Phenomena (Instruments: Fluorometer) Conditions for Fluorescence Lifetime Measurements Time-Resolved Protein Fluorescence Ultra-Short (picosecond) Fluorescence Lifetime Measurements Anisotropy Decays Time-Resolved Emission Spectra Advanced Techniques and Applications Multi-Photon Excitation (Ultra-Fast Pulsed Lasers) Stimulated Emission Depletion (Light Quenching) Total Internal Reflection Fluorescence (TIRF - Evanescent Wave) Metal Enhanced Fluorescence (MEF) Surface Plasmon Coupled Emission (SPCE) Fluorescence Correlation Spectroscopy (FCS) Single Molecule Detection (SMD) Fluorescence-Based Sensing