Main description:

This volume provides readers with a collection of the latest protocols used by researchers to study polyamines (PA). The chapters in this book cover various topics, such as quantification of different polyamines and conjugates, subcellular localization studies, transport, DNA methylation, ODC regulation, genetic and phenotyping analyses, genome-wide association mapping, polyamine applications and cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Polyamines: Methods and Protocols is a useful reference for researchers looking to advance and stimulate their knowledge of polyamines.

Contents:

Preface...
Table of Contents...
Contributing Authors...

1. Polyamines in the Context of Metabolic Networks
Wegi Wuddineh, Rakesh Minocha, and Subhash C. Minocha

2. Molecules for Sensing Polyamines and Transducing their Action in Plants
Tomonobu Kusano, GHM Sagor, and Thomas Berberich

3. Polyamine Metabolism Responses to Biotic and Abiotic Stress
Fernando M. Romero, Santiago J. Maiale, Franco R. Rossi, Maria Marina, Oscar A. Ruiz, and Andres Garriz

4. Thermospermine: An Evolutionary Ancient but Functionally New Compound in Plants
Taku Takahashi

5. Determination of Polyamines by Capillary Electrophoresis using Salicylaldehyde-5-Sulfonate as a Derivatizing Reagent
Takashi Kaneta

6. Detection of Thermospermine and Spermine by HPLC in Plants
Taku Takahashi, Ayaka Takano, and Jun-Ichi Kakehi

7. Development of Amine-Oxidase Based Biosensors for Spermine and Spermidine Analysis
Cristina Tortolini, Gabriele Favero, and Franco Mazzei

8. Identification of Branched-Chain Polyamines in Hyperthermophiles
Ryota Hidese, Wakao Fukuda, Masaru Niitsu, and Shinsuke Fujiwara

9. Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry
Laura Torras-Claveria, Jaume Bastida, Francesc Viladomat, and Antonio F. Tiburcio

10. Analysis of Glutathione in Biological Samples by HPLC Involving Pre-Column Derivatization with o-Phthalaldehyde
Yongqing Hou, Xilong Li, Zhaolai Dai, Zhenlong Wu, Fuller W. Bazer, and Guoyao Wu

11. Determination of Arginine and Ornithine Decarboxylase Activities in Plants
Ruben Alcazar and Antonio F. Tiburcio

12. Determination of S-Adenosylmethionine Decarboxylase Activity in Plants
Ruben Alcazar and Antonio F. Tiburcio

13. Determination of Copper Amine Oxidase Activity in Plant Tissues
Riccardo Angelini, Alessandra Cona, and Paraskevi Tavladoraki

14. Determination of di-/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay
Panagiotis N. Moschou

15. Pentamine as a Substrate for Measuring Spermine Oxidase Activity
Koichi Takao and Yoshiaki Sugita

16. Spectrophotometric Quantification of Reactive Oxygen, Nitrogen and Sulfur Species in Plant Samples
Chrystalla Antoniou, Andreas Savvides, Egli Georgiadou, and Vasileios Fotopoulos

17. Novel Route for Agmatine Catabolism in Aspergillus niger: 4-Guanidinobutyrase Assay
Tejaswani Saragadam and Narayan S.Punekar

18. Determination of Transglutaminase Activity in Plants
S. Del Duca, P.LR. Bonner, I. Aloisi, D. Serafini-Fracassini, and G. Cai

19. Procedures for ADC Immunoblotting and Immunolocalization for Transmission Electron Microscopy during Organogenic Nodule Formation in Hop
Ana Margarida Fortes and Jose M. Segui-Simarro

20. Analysis of the Intracellular Localization of Transiently Expressed and Fluorescently Labeled Copper-Containing Amine Oxidases, Diamine Oxidase and N-Methylputrescine Oxidase in Tobacco, using an Agrobacterium infiltration Protocol
Tsubasa Shoji

21. Techniques used for Functional Characterization of Polyamine Transporters
Claudio A. Pereira, Melisa Saye, Chantal Reigada, and Mariana R. Miranda

22. Quantitative Trait Loci for Root Growth Response to Cadaverine in Arabidopsis
Nicole M. Gibbs, Laura Vaughn Rouhana, and Patrick H. Masson

23. Methods Related to Polyamine Control of Cation Transport across Plant Membranes
Isaac Zepeda-Jazo and Igor Pottosin

24. Analysis of DNA Methylation Content and Patterns in Plants
Andreas Finke, Wilfried Rozhon, and Ales Pecinka

25. Investigating Ornithine Decarboxylase Post-Transcriptional Regulation via a Pulldown Assay using Biotinylated Transcripts
Anh Mai and Shannon L. Nowotarski

26. Analysis of Cotranslational Polyamine Sensing during Decoding of ODC Antiyme mRNA
R. Palanimurugan, Daniela Goedderz, Leo Kurian, and R. Jurgen Dohmen

27. Modulation of Protein Synthesis by Polyamines in Mammalian Cells
Keiko Kashiwagi, Yusuke Terui, and Kazuei Igarashi

28. Determination of Post-Translational Modifications by 2D-PAGE: Applications to Polyamines
Marta Bitrian, Antonio F. Tiburcio, and Ruben Alcazar

29. Generation of EMS-Mutagenized Populations of Arabidopsis thaliana for Polyamine Genetics
Kostadin E. Atanasov, Changxin Liu C, Antonio F. Tiburcio, and Ruben Alcazar

30. Transcriptome Analysis of PA Gain and Loss of Function Mutants
Francisco Marco and Pedro Carrasco

31. High-Throughput Phenotyping in Plant Stress Response: Methods and Potential Applications to Polyamine Field
D. Marko, N. Briglia, S. Summerer, A. Petrozza, F. Cellini, and R. Iannacone

32. Abiotic Stress Phenotyping of Polyamine Mutants
T. Berberich, GHM Sagor, and T. Kusano

33. Phenotypic and Genotypic Characterization of Mutant Plants in Polyamine Metabolism Genes during Pathogenic Interactions
F.R. Rossi, F.M. Romero, O.A. Ruiz, M. Marina, and A. Garriz

34. Real-Time In Vivo Monitoring of Reactive Oxygen Species in Guard Cells
Ky Young Park and Kalliopi A. Roubelakis-Angelakis

35. Genome-Wide Association Mapping Analyses Applied to Polyamines
Luis Barboza-Barquero, Paul Esker, and Ruben Alcazar

36. Polyamine Metabolism in Climacteric and Non-Climacteric Fruit Ripening
Ana Margarida Fortes and Patricia Agudelo-Romero

37. Application of Polyamines to Maintain Functional Properties in Stored Fruits
Maria Serrano and Daniel Valero

38. Acrolein: An Effective Biomarker for Tissue Damage Produced from Polyamines
Kazuei Igarashi, Takeshi Uemura, and Keiko Kashiwagi

39. Polyamines and Cancer
Elisabetta Damiani and Heather M. Wallace

40. Potential Applications of Polyamines in Agriculture and Plant Biotechnology
Antonio F. Tiburcio and Ruben Alcazar