Main description:

This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts.

Contents:

Neuromethods Series Preface...Preface...Table of Contents...Contributing Authors...
1. User's Guide to the Selection of Viral Vectors for Optogenetic StudiesK.R. Thompson and C. Towne
2. Cell Type-Specific Targeting Strategies for OptogeneticsOfer Yizhar and Antoine Adamantidis
3. Molecular Engineering of Channelrhodopsis for Enhanced Control over the Nervous SystemAndre Berndt
4. Optogenetic Control of Intracellular Signaling: Class II OpsinsErik Ellwardt and Raag D. Airan
5. Optogenetics in Stem Cell Research: Focus on the Central Nervous SystemJohannes Boltze and Albrecht Stroh
6. Optogenetic Applications in the Nematode Caenorhabditis elegansKatharina Elisabeth Fischer, Nathalie Alexandra Vladis, and karl Emanuel Busch
7. Optogenetic Interpellation of Behavior Employing Unrestrained Zebrafish LarvaeSoojin Ryu and Rodrigo J. De Marco
8. Combining Optogenetics with MEA, Depth-Resolved LFPs and Assessing the Scope of Optogenetic Network ModulationJeng-Wei Yang, Pierre-Hugues Prouvot, Albrecht Stroh, and Heiko J. Luhmann
9. Concepts of All-Optical PhysiologyJan Doering, Ting Fu, Isabelle Arnoux, and Albrecht Stroh
10. Two-Photon Optogenetics by Computer-Generated HolographyEirini Papagiakoumou, Emiliano Ronzitti, I-Wen Chen, Marta Gajowa, Alexis Picot, and Valentina Emiliani
11. Optophysiology and Behavior in Rodents and Non-Human PrimatesGolan Karvat and Ilka Diester
12. Employing Optogenetics in Memory ResearchLimor Regev and Inbal Goshen
13. Towards Opto-Magnetic Physiology: Concepts and Pitfalls of ofMRIMiriam Schwalm, Eduardo Rosales-Jubal, and Albrecht Stroh
14. Optogenetics: Lighting a Path from the Laboratory to the ClinicHannah K. Kim, Allyson L. Alexander, and Ivan Soltesz