Main description:

This detailed book arrives as there is an increasing need for multiplex biomarker readouts for improved clinical management and to support the development of new drugs by pharmaceutical companies, due to continuous technical developments and new insights into the high complexity of many diseases. Chapters explore the basic technology platforms being applied in the fields of genomics, proteomics, transcriptomics, metabolomics, and imaging, which are currently the methods of choice in multiplex biomarker research. The book also describes the chief medical areas in which the greatest progress has been made and highlight areas where further resources are required. Written for the highly successful Methods in Molecular Biology series, methodology chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Multiplex Biomarker Techniques: Methods and Applications serves as an ideal resource for a wide variety of researchers interested in these vital multiplex techniques.

Contents:

Part I: Reviews

1. Application of Multiplex Biomarker Approaches to Accelerate Drug Discovery and Development

Hassan Rahmoune and Paul C. Guest

2. The Application of Multiplex Biomarker Techniques for Improved Stratification and Treatment of Schizophrenia Patients

Johann Steiner, Paul C. Guest, Hassan Rahmoune, and Daniel Martins-de-Souza

3. Multiplex Biomarker Approaches in Type 2 Diabetes Mellitus Research

Susan E. Ozanne, Hassan Rahmoune, Daniel Martins-de Souza, and Paul C. Guest

4. LC-MSE, Multiplex MS/MS, Ion Mobility, and Label-Free Quantitation in Clinical Proteomics

Gustavo Henrique Martins Ferreira Souza, Paul C. Guest, and Daniel Martins-de-Souza

5. Phenotyping Multiple Subsets of Immune Cells In Situ in FFPE Tissue Sections: An Overview of Methodologies

James R. Mansfield

Part II: Statistical Considerations

6. Identification and Clinical Translation of Biomarker Signatures: Statistical Considerations

Emanuel Schwarz

7. Opportunities and Challenges of Multiplex Assays: A Machine Learning Perspective

Junfang Chen and Emanuel Schwarz

Part III: Protocols

8. Multiplex Analyses Using Real-Time Quantitative PCR

Steve F.C. Hawkins and Paul C. Guest

9. Multiplex Analysis Using cDNA Transcriptomic Profiling

Steve F.C. Hawkins and Paul C. Guest

10. Multiplex Single Nucleotide Polymorphism Analyses

Steve F.C. Hawkins and Paul C. Guest

11. Pulsed SILAC as a Proteomic Approach for miRNA Targets Identification in Cell Culture

Daniella E. Duque-Guimaraes, Juliana de Almeida-Faria, Thomas Prates Ong, and Susan E. Ozanne

12. Blood Bio-Sampling Procedures for Multiplex Biomarkers Studies

Paul C. Guest and Hassan Rahmoune

13. Multiplex Immunoassay Profiling

Laurie Stephen

14. Multiplex Sequential Immunopreciptation of Insulin Secretory Granule Proteins from Radiolabeled Pancreatic Islets

Paul C. Guest

15. Two Dimensional Gel Electrophoresis of Insulin Secretory Granule Proteins from Biosynthetically-Labeled Pancreatic Islets

Paul C. Guest

16. Depletion of Highly Abundant Proteins of the Human Blood Plasma: Applications in Proteomics Studies of Psychiatric Disorders

Sheila Garcia, Paulo A. Baldasso, Paul C. Guest, and Daniel Martins-de-Souza

17. Simultaneous Two-Dimensional Difference Gel Electrophoresis (2D-DIGE) Analysis of Three Distinct Proteomes

Adriano Aquino, Paul C. Guest, and Daniel Martins-de Souza

18. Selective Reaction Monitoring for Quantitation of Cellular Proteins

Vitor M. Faca

19. Characterization of a Protein Interactome by Co-Immunoprecipitation and Shotgun Mass Spectrometry

Giuseppina Maccarrone, Juan Jose Bonfiglio, Susana Silberstein, Christoph W. Turck, and Daniel Martins-de-Souza

20. Using 15N-Metabolic Labeling for Quantitative Proteomic Analyses

Maccarrone Giuseppina, Alon Chen, and Michaela D. Filiou

21. Multiplex Measurement of Serum Folate Vitamers by UPLC-MS/MS

Sarah Meadows

22. UPLC-MS/MS Determination of Deuterated 25-Hydroxyvitamin D (d3-25OHD3) and Other Vitamin D Metabolites for the Measurement of 25OHD Half-Life

Shima Assar, Inez Schoenmakers, Albert Koulman, Ann Prentice, and Kerry S. Jones

23. iTRAQ-Based Shotgun Proteomics Approach for Relative Protein Quantification

Erika Velasquez Nunez, Gilberto Barbosa Domont, and Fabio Cesar Sousa Nogueira

24. 1H NMR Metabolomic Profiling of Human and Animal Blood Serum Samples

Joao G.M. Pontes, Antonio J.M. Brasil, Guilherme C.F. Cruz, Rafael N. de Souza, and Ljubica Tasic

25. Lab-on-a-Chip Multiplex Assays

Harald Peter, Julia Wienke, and Frank F. Bier

26. Multiplex Smartphone Diagnostics

Juan L. Martinez-Hurtado, Ali K. Yetisen, and Seok H. Yun

27. Development of a User-Friendly App for Assisting Anticoagulation Treatment

Johannes Vegt

Part IV: Future Directions

28. Multiplex Biomarker Approaches to Enable Point-of-Care Testing and Personalized Medicine

Paul C. Guest