Main description:

Written and edited by recognized experts in the field, the new "Artech House Methods in Bioengineering book series" offers detailed guidance on authoritative methods for addressing specific bioengineering laboratory challenges. Offering a highly practical presentation of each topic, each book provides research engineers, scientists, and students with step-by-step procedures, clear examples, and effective ways to overcome possible problems. This first-of-its-kind volume addresses the important challenge of organ preservation and reengineering. This book presents cutting-edge techniques for damaged livers and hearts via normothermic perfusion, hypothermic machine perfusion for the liver, kidney and pancreas, and imaging techniques to assess the viability of injured kidneys. Professionals and researchers also find methods to decellularize whole organs to create scaffolds for tissue engineering. Moreover, this book presents an approach to enhancing hepatocyte recovery from marginal livers.

Contents:

Introduction. Preservation - Normothermic Machine Perfusion of the Liver. Heart Preservation. Hypothermic Machine Perfusion of the Kidneys. Methods of Cardiac Oxygen Persufflation. Vascular Oxygen Persufflation for Preservation and Reconditioning of Marginal Liver Grafts. Use of Optical Imaging and Spectroscopy in Assessment of Organ Perfusion. Reengineering - Preparation of a Transplantable Liver Graft by Recellularization of Decellularized Whole Organs. Engineering Lung Tissue. Ex Vivo Perfusion of Rat Liver Grafts for Hepatocyte Retrieval.