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Histochemistry of Single Molecules
Methods and Protocols
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Main description:

This detailed volume explores numerous histochemical techniques through a series of lab-tested protocols for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets. It begins with a section on vital histochemistry and continues with chapters on histochemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital histochemistry, lectin histochemistry, and DNA fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Histochemistry of Single Molecules: Methods and Protocols aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.


Contents:

Part I: Vital Histochemistry

1. Single Cell Cytochemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes

Anna L. Peters and Cornelis J.F. van Noorden

2. Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues

Anna C. Croce and Giovanni Bottiroli

3. Enzyme-Histochemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree

Vittorio Bertone, Eleonora Tarantola, and Isabel Freitas

4. Histochemical Demonstration of Tripeptidyl Aminopeptidase I

Mashenka B. Dimitrova, Dimitrinka Y. Atanasova, and Nikolai E. Lazarov

5. Enzyme Histochemistry for Functional Histology in Invertebrates

Francesca Cima

Part II: Lectin Histochemistry

6. Lectin Histochemistry: Historical Perspectives, State of the Art, and the Future

Susan A. Brooks

7. Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads

Ellie-May Beaman, David R.F. Carter, and Susan A. Brooks

8. Lectin Histochemistry for Metastasizing and Non-Metastasizing Cancer Cells

Gerrit Wolters-Eisfeld and Udo Schumacher

9. The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

Vicente Seco-Rovira, Ester Beltran-Frutos, Jesus Hernandez-Martinez, Concepcion Ferrer, and Luis Miguel Pastor

Part III: Protein Histochemistry

10. Heat-Induced Antigen Retrieval in Immunohistochemistry: Mechanisms and Applications

Shuji Yamashita and Osamu Katsumata

11. Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain

Sara Bonzano and Silvia De Marchis

12. Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration

Nicolas A. Dumont and Michael A. Rudnicki

13. Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissue

Sabina Berezowska and Jose A. Galvan

Part IV: Lipid Histochemistry

14. Tissue Fixation and Processing for the Histological Identification of Lipids

Victor Carriel, Fernando Campos, Jose Aneiros-Fernandez, and John A. Kiernan

15. Staining Methods for Normal and Regenerative Myelin in the Nervous System

Victor Carriel, Antonio Campos, Miguel Alaminos, Stefania Raimondo, and Stefano Geuna

16. Nile Red Staining of Neutral Lipids in Yeast

Kerry Ann Rostron and Clare Louise Lawrence

17. Staining of Lipid Droplets with Monodansylpentane

Bo-Hua Chen, Huei-Jiun Yang, He-Yen Chou, Guang-Chao Chen, and Wei Yuan Yang

Part V: Nuclear Histochemistry

18. Fluorochromes for DNA Staining and Quantitation

Giuliano Mazzini and Marco Danova

19. Osmium Ammine for Staining DNA at Electron Microscopy

Irene Masiello and Marco Biggiogera

20. DNA Labeling at Electron Microscopy

Nicolas Thelen and Marc Thiry

21. Visualizing RNA at Electron Microscopy by Terbium Citrate

Marco Biggiogera and Irene Masiello

22. Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks

Elva I. Cortes-Gutierrez, Jose Luis Fernandez, Martha I. Davila-Rodriguez, Carmen Lopez-Fernandez, and Jaime Gosalvez

Part VI: Plant Histochemistry

23. Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogues of Lamins

Malgorzata Ciska and Susana Moreno Diaz de la Espina

24. Histochemical Analysis of Plant Secretory Structures

Diego Demarco

25. A Histochemical Technique for the Detection of Annonaceous Acetogenins

Guillermo Laguna-Hernandez, Alicia Enriqueta Brechu-Franco, Ivan De la Cruz-Chacon, and Alma Rosa Gonzalez- Esquinca


PRODUCT DETAILS

ISBN-13: 9781493967872
Publisher: Springer (Humana Press Inc.)
Publication date: February, 2017
Pages: None
Weight: 8196g
Availability: Available
Subcategories: Biochemistry

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