MORE ABOUT THIS BOOK
Main description:
This detailed volume introduces the reader to current methods in glaucoma research, in particular those that make use of animal disease models. Given that the retinal ganglion cells damaged and destroyed by glaucoma do not regenerate after lowering the intraocular pressure, new therapeutic approaches that protect ganglion cells directly is a vital research goal that this volume's contents aim to aid scientists in developing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Glaucoma: Methods and Protocols serves as a useful guide for both ophthalmologists in clinical practice and researchers in the field.
Contents:
1. Use of Animal Models and Techniques in Glaucoma Research: Introduction
Harry A. Quigley
2. Hypertonic Saline Injection Model of Experimental Glaucoma in Rats
John C. Morrison, Elaine C. Johnson, and William O. Cepurna
3. The Microbead Occlusion Model of Ocular Hypertension in Mice
David J. Calkins, Wendi S. Lambert, Cathryn R. Formichella, William M. McLaughlin, and Rebecca M. Sappington
4. Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods
Elena Vecino, Haritz Urcola, and Sansar C. Sharma
5. Non-Invasive Intraocular Pressure Measurement in Animals Models of Glaucoma
Yan Hu and John Danias
6. High Throughput Binocular Pattern Electroretinograms in the Mouse
Tsung-Han Chou, Jonathon Toft-Nielsen, and Vittorio Porciatti
7. Visual Evoked Potentials in Glaucoma and Alzheimer's Disease
Elisa Cerri, Carlotta Fabiani, Chiara Criscuolo, and Luciano Domenici
8. Investigation of the Functional Retinal Output Using Microelectrode Arrays
Gunther Zeck
9. Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling
Anna Trzeciecka, Padmanabhan Pattabiraman, Maria Carmen Piqueras, Carol Toris, and Sanjoy K. Bhattacharya
10. Sphingolipid Analysis of Aqueous Humor
Anna Trzeciecka, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, and Sanjoy K. Bhattacharya
11. Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion
J. Cameron Millar, Tien N. Phan, and Iok-Hou Pang
12. Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models
Prabhavathi Maddineni, Ramesh B. Kasetti, and Gulab S. Zode
13. Quantification of Scleral Biomechanics and Collagen Fiber Alignment
Ian C. Campbell, Joseph M. Sherwood, Darryl R. Overby, Bailey G. Hannon, A. Thomas Read, Julia Raykin, and C. Ross Ethier
14. Biolistic Labeling of Retinal Ganglion Cells
Luca Della Santina and Yvonne Ou
15. Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma
Samuel D. Crish and Brett R. Schofield
16. In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration
Yuqin Yin and Larry I. Benowitz
17. 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues
Hongli Yang, Juan Reynaud, Howard Lockwood, Galen Williams, Christy Hardin, Luke Reyes, Stuart Gardiner, and Claude F. Burgoyne
18. Visualizing Astrocytes of the Optic Nerve
Daniel Sun
19. Investigation of MicroRNA Expression in Experimental Glaucoma
Hari Jayaram, Diana C. Lozano, Elaine C. Johnson, and John C. Morrison
20. Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head
Diana C. Lozano, Dongseok Choi, Hari Jayaram, John C. Morrison, and Elaine C. Johnson
21. Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve
Hee Joo Choi, Rui Wang, and Tatjana C. Jakobs
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2017
Pages: None
Weight: 1135g
Availability: Available
Subcategories: General Issues, Ophthalmology and Optometry