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Glaucoma
Methods and Protocols
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Main description:

This detailed volume introduces the reader to current methods in glaucoma research, in particular those that make use of animal disease models. Given that the retinal ganglion cells damaged and destroyed by glaucoma do not regenerate after lowering the intraocular pressure, new therapeutic approaches that protect ganglion cells directly is a vital research goal that this volume's contents aim to aid scientists in developing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Glaucoma: Methods and Protocols serves as a useful guide for both ophthalmologists in clinical practice and researchers in the field.


Contents:

1. Use of Animal Models and Techniques in Glaucoma Research: Introduction

Harry A. Quigley

2. Hypertonic Saline Injection Model of Experimental Glaucoma in Rats

John C. Morrison, Elaine C. Johnson, and William O. Cepurna

3. The Microbead Occlusion Model of Ocular Hypertension in Mice

David J. Calkins, Wendi S. Lambert, Cathryn R. Formichella, William M. McLaughlin, and Rebecca M. Sappington

4. Ocular Hypertension/Glaucoma in Minipigs: Episcleral Veins Cauterization and Microbead Occlusion Methods

Elena Vecino, Haritz Urcola, and Sansar C. Sharma

5. Non-Invasive Intraocular Pressure Measurement in Animals Models of Glaucoma

Yan Hu and John Danias

6. High Throughput Binocular Pattern Electroretinograms in the Mouse

Tsung-Han Chou, Jonathon Toft-Nielsen, and Vittorio Porciatti

7. Visual Evoked Potentials in Glaucoma and Alzheimer's Disease

Elisa Cerri, Carlotta Fabiani, Chiara Criscuolo, and Luciano Domenici

8. Investigation of the Functional Retinal Output Using Microelectrode Arrays

Gunther Zeck

9. Quantitative Proteomic Analysis of Human Aqueous Humor Using iTRAQ 4plex Labeling

Anna Trzeciecka, Padmanabhan Pattabiraman, Maria Carmen Piqueras, Carol Toris, and Sanjoy K. Bhattacharya

10. Sphingolipid Analysis of Aqueous Humor

Anna Trzeciecka, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, and Sanjoy K. Bhattacharya

11. Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion

J. Cameron Millar, Tien N. Phan, and Iok-Hou Pang

12. Methods for Analyzing Endoplasmic Reticulum Stress in the Trabecular Meshwork of Glaucoma Models

Prabhavathi Maddineni, Ramesh B. Kasetti, and Gulab S. Zode

13. Quantification of Scleral Biomechanics and Collagen Fiber Alignment

Ian C. Campbell, Joseph M. Sherwood, Darryl R. Overby, Bailey G. Hannon, A. Thomas Read, Julia Raykin, and C. Ross Ethier

14. Biolistic Labeling of Retinal Ganglion Cells

Luca Della Santina and Yvonne Ou

15. Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma

Samuel D. Crish and Brett R. Schofield

16. In Vitro and In Vivo Methods for Studying Retinal Ganglion Cell Survival and Optic Nerve Regeneration

Yuqin Yin and Larry I. Benowitz

17. 3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues

Hongli Yang, Juan Reynaud, Howard Lockwood, Galen Williams, Christy Hardin, Luke Reyes, Stuart Gardiner, and Claude F. Burgoyne

18. Visualizing Astrocytes of the Optic Nerve

Daniel Sun

19. Investigation of MicroRNA Expression in Experimental Glaucoma

Hari Jayaram, Diana C. Lozano, Elaine C. Johnson, and John C. Morrison

20. Utilizing RNA-Seq to Identify Differentially Expressed Genes in Glaucoma Model Tissues, Such as the Rodent Optic Nerve Head

Diana C. Lozano, Dongseok Choi, Hari Jayaram, John C. Morrison, and Elaine C. Johnson

21. Single-Cell Dissociation and Characterization in the Murine Retina and Optic Nerve

Hee Joo Choi, Rui Wang, and Tatjana C. Jakobs


PRODUCT DETAILS

ISBN-13: 9781493974061
Publisher: Springer (Humana Press Inc.)
Publication date: December, 2017
Pages: None
Weight: 1135g
Availability: Available
Subcategories: General Issues, Ophthalmology and Optometry

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