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MORE ABOUT THIS BOOK
Main description:
This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons. The chapters in this book are divided into 5 parts, and cover the following topics: principles and approaches for discovery of somatic mosaicism in the brain, aneuploidy and ploidy variation, DNA copy number variation, LINE-1 retrotransposition, and genetic and genomic mosaicism in aging and disease. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and authoritative, Genomic Mosaicism in Neurons and Other Cell Types is a valuable resource for learning about the latest techniques for the analysis of genome and genetic mosaicism in vertebrate neurons.
Contents:
Neuromethods Series Preface...
Preface...
Table of Contents...
Contributing Authors...
Part I Introduction
1. Principles and Approaches for Discovery and Validation of Somatic Mosaicism in the Human Brain
Alexej Abyzov, Alexander E. Urban, and Flora M. Vaccarino
Part II Aneuploidy and Ploidy Variation
2. FISH-Based Assays for Detecting Genomic (Chromosomal) Mosaicism in Human Brain Cells
Yuri B. Yurov, Svetlana G. Vorsanova, Ilia V. Soloviev, Alexei M. Ratnikov, and Ivan Y. Iourov
3. Flow Cytometric and Sorting Analyses for Nuclear DNA Content, Nucleotide Sequencing and Interphase FISH
Gwendolyn E. Kaeser and Jerold Chun
4. Flow Cytometric Quantification, Isolation, and Subsequent Epigenetic Analysis of Tetraploid Neurons
Noelia Lopez-Sanchez, Iris Patino-Parrado, and Jose M. Frade
5. A Cytomic Approach towards Genomic Individuality of Neurons
Thomas Arendt, Birgit Belter, Martina K. Bruckner, Uwe Ueberham, Markus Morawski, and Attila Tarnok
Part III DNA Copy Number Variation
6. Single Cell CNV Detection in Human Neuronal Nuclei
Margaret B. Wierman, Ian E. Burbulis, William D. Chronister, Stefan Bekiranov, and Michael J. McConnell
7. Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) for the Analysis of DNA Copy Number Variation
Chenghang Zong
8. Competitive PCR for Copy Number Assessment by Restricting dNTPs
Luming Zhou, Robert A. Palais, Yotam Ardon, and Carl T. Wittwer
9. Using Cloning to Amplify Neuronal Genomes for Whole Genome Sequencing and Comprehensive Mutation Detection and Validation
Jennifer L. Hazen, Michael A. Duran, Ryan P. Smith, Alberto R, Rodriguez, Greg S. Martin, Sergey Kupriyanov, Ira M. Hall, and Kristin K. Baldwin
Part IV LINE-1 Retrotransposition
10. Analysis of LINE-1 Retrotransposition in Neural Progenitor Cells and Neurons
Angela Macia and Alysson R. Muotri
11. Estimation of LINE-1 Copy Number in the Brain Tissue and Isolated Neuronal Nuclei
Miki Bundo, Tadafumi Kato, and Kazuya Iwamoto
12. Analysis of Somatic LINE-1 Insertions in Neurons
Francisco J. Sanchez-Luque, Sandra R. Richardson, and Geoffrey J. Faulkner
13. Single-Cell Whole Genome Amplification and Sequencing to Study Neuronal Mosaicism and Diversity
Patrick J. Reed, Meiyan Wang, Jennifer A. Erwin, Apua C.M. Paquola, and Fred H. Gage
Part V Genetic and Genomic Mosaicism in Aging and Disease
14. FISH Analysis of Aging-Associated Aneuploidy in Neurons and Non-Neuronal Brain Cells
Grasiella A. Andriani and Cristina Montagna
15. Genomic Analysis and In Vivo Functional Validation of Brain Somatic Mutations Leading to Focal Cortical Malformations
Jae Seok Lim and Jeong Ho Lee
16. Using Fluorescence In Situ Hybridization (FISH) Analysis to Measure Chromosome Instability and Mosaic Aneuploidy in Neurodegenerative Diseases
Julbert Caneus, Antoneta Granic, Heidi J. Chial, Huntington Potter
17. Identification of Low Allele Frequency Mosaic Mutations in Alzheimer Disease
Carlo Sala Frigerio, Mark Fiers, Thierry Voet, and Bart De Strooper
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: September, 2017
Pages: 380
Weight: 8928g
Availability: Available
Subcategories: Neuroscience