Telomerase, an enzyme that maintains telomeres and endows eukaryotic cells with immortality, was first discovered in tetrahymena in 1985. In 1990s, it was proven that this enzyme also plays a key role in the infinite proliferation of human cancer cells. Now telomere and telomerase are widely accepted as important factors involved in cancer biology, and as promising diagnostic tools and therapeutic targets. Recently, role of telomerase in “cancer stem cells” has become another attractive story. Until now, there are several good books on telomere and telomerase focusing on biology in ciliates, yeasts, and mouse or basic sciences in human, providing basic scientists or students with updated knowledge.
This book will focus on telomere and telomerase in human cancers and may provide medical doctors, pathologists, and translational cancer researchers with a deep understanding of these unique and fascinating molecules, as well as hot topics in clinical applications
Telomeres and telomerase are the pivotal players that destine cellular senescence and immortalization. Telomere shortening due to "End-replication problem" in each cell cycle is a universal phenomenon for all eukaryotic cells with linear chromosomes. Its compensation by telomerase is also common from yeast to human. However, the length of telomeres, distribution of telomerase positive cells, and consequence of telomerase inhibition are different among species. "Telomeres and Telomerase in Cancers", edited by Keiko Hiyama, focuses on "Human" telomeres and telomerase, from basic biology and hypothesis in human normal cells and cancer cells to clinical application as diagnostic tools and therapeutic targets in anti-cancer strategy. Cutting-edge experimental protocols for their evaluation are also included. This coverage will enable medical oncologists and students as well as basic researchers to capture the up-to-date entire picture of human telomeres and telomerase in cancer.
Preface Contributors Part I. Basic background 1 Telomeres and Telomerase in Human Keiko Hiyama, Eiso Hiyama, and Jerry W. Shay 2 Telomere Binding Proteins in Human Nadya Dimitrova 3 Regulation of Telomerase through Transcriptional and Post-Translational Mechanisms Amy N. Depcrynski, Patrick C. Sachs, Lynne W. Elmore, and Shawn E. Holt 4 Telomere Dysfunction and the DNA Damage Response Malissa C. Diehl, Lynne W. Elmore, Shawn E. Holt 5 Alternative Lengthening of Telomeres (ALT) in Human Cells Hilda A. Pickett and Roger R. Reddel 6 Mouse model: Telomeres and Telomerase in Stem Cell and Cancer Xin Huang, Zhenyu Ju Part II Telomeres and telomerase in human cancers 7 Role of Telomeres and Telomerase in Cancer Keiko Hiyama, Eiso Hiyama, Keiji Tanimoto, Masahiko Nishiyama 8 Diagnostic Value 1: Solid Tumors Eiso Hiyama and Keiko Hiyama 9 Diagnostic Value 2: Hematopoietic Malignancies Junko H. Ohyashiki and Kazuma Ohyashiki 10 Therapeutic Targets and Drugs 1: Telomerase and Telomerase Inhibitors Brittney-Shea Herbert and Erin M. Goldblatt 11 Therapeutic Targets and Drugs 2: G-quadruplex and G-quadruplex Inhibitors Chandanamali Punchihewa and Danzhou Yang 12 Therapeutic Targets and Drugs 3: Tankyrase 1, Telomere Binding Proteins, and Inhibitors Hiroyuki Seimiya and Takashi Tsuruo 13 Therapeutic Targets and Drugs 4: Telomerase-specific Gene and Vector-based Therapies for Human Cancer Toshiyoshi Fujiwara, Yasuo Urata, and Noriaki Tanaka Part III Experimental protocols 14 Protocol 1: Telomerase Activity and Telomerase Expression Eiso Hiyama 15 Protocol 2: Importance and Methods of Telomere G-tail Length Quantification Akira Shimamoto, Eriko Aoki, Angie M Sera, Hidetoshi Tahara 16 Protocol 3: Detection of Alternative Lengthening of Telomeres (ALT) Wei-Qin Jiang, Jeremy D. Henson, Axel A. Neumann, and Roger R. Reddel Index