While the genomic revolution has quickly led to the deposit of more than 30,000 structures in the protein data bank (PDB), less than one percent of those contributions represent membrane proteins despite the fact that membrane proteins constitute some 20 percent of all proteins. This discrepancy becomes significantly troublesome when it is coupled with the fact that 60 percent of current drugs are based on targeting this group of proteins, a trend that does not seem likely to reverse. Structural Genomics on Membrane Proteins provides an excellent overview on novel research in bioinformatics and modeling on membranes, as well as the latest technological developments being employed in expression, purification, and crystallography to obtain high-resolution structures on membrane proteins. This cutting-edge work also explains the difficulties facing researchers-both technical and ethical-that have slowed the process. Structural Genomics on Membrane Proteins provides researchers with an unprecedented look at the novel technologies that will ultimately allow them to conquer the last frontier in structural biology, leading to accelerated breakthroughs in drug discovery.
Introduction; Kenneth Lundstrom Bioinformatics in Membrane Protein Analysis; Bengt Persson Prokaryotic Membrane Transport Proteins: Amplified Expression and Purification; Joanne Clough, Massoud Saidijam, Kim Bettaney, Gerda Szakonyi, Simon Patching, Johan Meuller, Shun'ichi Suzuki, Keigo Shibayama, Mark Bacon, Emma Barksby, Marie Groves, Richard Herbert, Mary Phillips-Jones, Alison Ward, Frank Gunn-Moore, John O'Reilly, Nick Rutherford, Roslyn Bill, and Peter Henderson Membrane Protein Production Strategies for Structural Genomics; Said Eshaghi and Par Nordlund Refolding of Membrane Proteins for Large-Scale Production; Hans Kiefer, Thomas Ostermann, and Monika Bahner Crystallization of Membrane Proteins; Alastair T. Gardiner, June Southall, and Richard J. Cogdell Signaling through Membrane Proteins; J. Robert Bostwick and Deborah S. Hartman Expression of Membrane Proteins in Yeasts; Christoph Reinhart and Christoph Krettler Expression of Functional Membrane Proteins in the Baculovirus-Insect Cell System: Challenges and Developments; Giel J.C.G.M. Bosman and Willem J. De Grip Expression of Membrane Proteins in Mammalian Cells; Kenneth Lundstrom Solubilization and Purification of Membrane Proteins; Bernadette Byrne and Mika Jormakka Fluorescent Labelling of Membrane Proteins in Living Cells; Ruud Hovius, Bruno H. Meyer, Emmanuel G. Guignet, and Horst Vogel Membrane Protein NMR; Xiang-Qun (Sean) Xie Miniaturization of Structural Biology Technologies - From Expression to Biophysical Analyses; Enrique Abola, Peter Kuhn and Raymond C. Stevens Electron Microscopy and Atomic Force Microscopy of Reconstituted Membrane Proteins; Andreas Engel Structural Genomics Networks for Membrane Proteins; Kenneth Lundstrom Molecular Modelling of Membrane Proteins; Slawomir Filipek and Anna Modzelewska Toward Structural Bases for GPCR Ligand binding: A Path for Drug Discovery; Geoffrey F. Stamper, Sabrina A. Beretta, Alla Korepanova, Mulugeta Mamo, and Mark L. Chiu Index