These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant.
THE REDOX BIOCHEMISTRY OF HYDROGEN SULFIDE 1. Investigating the Role of H2S in 4-HNE Scavenging Hilde Laggner and Bernhard M.K. Gmeiner 2. Inhalation Exposure Model of Hydrogen Sulfide (H2S)-Induced Hypometabolism in the Male Sprague-Dawley Rats Asaf Stein, David W. Kraus, Jeannette E. Doeller and Shannon M. Bailey MECHANISMS OF H2S CELL SIGNALING AND TRANSCRIPTIONAL PATHWAYS 3. Use of the "Tag-SwitchÂ Method for the Detection of Protein S-Sulfhydration Chung-Min Park, Igor Macinkovic, Milos R. Filipovic, Ming Xian 4. Real-Time Assays for Monitoring the Influence of Sulfide and Sulfane Sulfur Species on Protein Thiol Redox States Romy Greiner and Tobias P. Dick 5. Sulfhydration of H2S Protein Targets Bindu D. Paul and Solomon H. Snyder H2S IN CELL SIGNALING IN THE CARDIOVASCULAR AND NERVOUS SYSTEM AND INFLAMMATORY PROCESSES 6. Intravital Microscopic Methods to Evaluate Anti-inflammatory Effects and Signaling Mechanisms Evoked by Hydrogen Sulfide Mozow Y. Zuidema and Ronald J. Korthuis 7. Attenuation of Inflammatory Responses by Hydrogen Sulfide (H2S) in Ischemia / Reperfusion Injury Neel R. Sodha and Frank W. Sellke 8. CD47-dependent Regulation of H2S Biosynthesis and Signaling in T Cells Sukhbir Kaur, Anthony L. Schwartz, Thomas W. Miller and David D. Roberts 9. Anti-Inflammatory and Cytoprotective Properties of Hydrogen Sulfide Burcu Gemici and & John L. Wallace 10. H2S and Substance P in Inflammation Madhav Bhatia 11. Role of Hydrogen Sulfide (H2S) in Brain Synaptic Remodeling Pradip Kumar Kamat, Anuradha Kalani and Neetu Tyagi H2S IN PLANTS 12. Detection of Thiol Modifications by Hydrogen Sulfide Williams, E., Pead, S., Whiteman, M., Wood, M.E., Wilson, I.D., Ladomery, M.R., Teklic, T., Lisjak, M. and Hancock, J.T. 13. Analysis of Some Enzymes Activities of Hydrogen Sulfide Metablism in Plants Zhong-Guang Li 14. Sulfide Detoxification in Plant Mitochondria Hannah Birke, Tatjana M. Hildebrandt, Markus Wirtz and Rudiger Hell MOLECULAR HYDROGEN 15. Molecular Hydrogen as a Novel Antioxidant: Overview of the Advantages of Hydrogen for Medical Applications Shigeo Ohta