Extracellular Glycolipids of Yeasts: Biodiversity, Biochemistry, and Prospects provides a comprehensive view of the biochemistry, biological activity, and practical application of extracellular glycolipids of yeast. This book brings much-needed clarity to the complex topic of glycolipids and streamlines the rather confusing terminology used for glycolipids. It also provides a wealth of modern data on their composition, structure and properties, biosynthetic pathways, methods of isolation and identification, antifungal activity, and mechanisms of action. Studies of extracellular glycolipids of yeast now draw the attention of researchers in life science and biotechnology due to numerous recently revealed biological properties of these compounds. These compounds are scientifically and practically promising in medicine and agriculture due to their biosurfactant and fungicidal properties, as well as a number of other biological activities. Extracellular Glycolipids of Yeasts gives researchers studying biochemistry of microorganisms and related biologically active compounds a much-needed guide to the basic data that will aid in these increasingly generative pursuits.
IntroductionChapter 1: Occurrence and structure of fungal extracellular glycolipids1.1. Glycolipid secretion in eumycetes 1.2. The structures of extracellular glycolipids of eumycetes 1.2.1. Cellobiose lipids1.2.2. Mannosylerythritol lipids, MELs1.2.3. Sophorolipids1.2.4. Rare extracellular fungal glycolipids Chapter 2: Methods of study of extracellular glycolipids 2.1. The culture media and methods to increase the yield of extracellular glycolipids 2.2. Purification methods2.3. Structure study by NRM-spectroscopy and mass-spectrometry2.4. Methods for studying physical and chemical properties, antifungaland membrane-damaging activitiesChapter 3: Physical and chemical properties of extracellular glycolipids3.1. Solubility3.2. Stability3.3. Molecular masses3.4. Surface properties3.5. Interaction with artificial membranesChapter 4: Biological activities of extracellular yeast glycolipids4.1. Antifungal activity of cellobiose lipids4.1.1. Discovery of antifungal activity of cellobiose lipids4.1.2. Antifungal activities and structure peculiarities4.2. Membrane - damaging activity of cellobiose lipids4.3. Biological activities of MELs and sophorolipidsChapter 5: Biosynthesis and degradation of extracellular yeast glycolipids5.1. Biosynthesis of cellobiose lipids5.2. Biosynthesis of sophorolipids5.3. Biosynthesis of MELs5.4. Degradation of extracellular yeast glycolipidsChapter 6: Applied prospects of cellobiose lipids, mannosylerythritol lipids, and sophorolipids6.1. Membrane-damaging agents for research purposes 6.2. Prospects for medicine, environmental protection, agriculture and industry6.3. Commercial products containing yeast extracellular glycolipids7. Supplement: selected techniques of purification and assay of extracellular yeast glycolipids 7.1. Culture media7.2. Chromatography 7.5. Fungicide activity assay7.6. Membrane-damaging activity assay8. References