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Experimental Surgical Models in the Laboratory Rat
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Main description:

The design of an adequate surgical model, like the choice of the animal model itself, is extremely important for obtaining reliable valuable data. Written for biomedical researcher this manual on the surgery of the rat summarizes a series of techniques that have been applied at the Bone Biology Laboratories of the Medical Science University of Rosario. Stopping short of an exhaustive analysis of the techniques, the manual describes the basis of each surgical technique, the necessary materials including catheters, precautions to consider, the methodology to apply and the possible results to be obtained. The descriptions of each technique appear with diagrams and pictures.


Contents:

Section I: Introduction Bioethics and Animal Care, Lucas R. M. Brun and Veronica Di Loreto Control of Stress and Distress, Maria F. Landoni Management of Pain in Laboratory Animals, Maria F Landoni Anesthesia and Analgesia, Veronica E. Di Loreto, Alfredo Rigalli, and Lucas F. de Candia Euthanasia, Alfredo Rigalli Antibiotics, Veronica Di Loreto and Alfredo Rigalli Section II: General Procedures Operating Theatre, Veronica Di Loreto and Alfredo Rigalli Rat Identification, Veronica Di Loreto and Alfredo Rigalli Antisepsis, Sterilization, and Asepsis, Diego Holotte,- Jesica Nipoti, Maria Julia Pretini, and Veronica Di Loreto Suture, Aneley Traverso and Alfredo Rigalli Substances Administration, Maela Lupo, Veronica Di Loreto, and Alfredo Rigalli Samples, Veronica Di Loreto, Laura Pera, and Alfredo Rigalli Section III: Catheterism and Cannulation Catheterism of the Femoral Artery and Vein, Veronica Di Loreto and Alfredo Rigalli Cardiac Catheterization, Cristina Lorenzo Carrion, Laura Krieger, Manuel Rodriguez, and Martin Donato Cannulation of the Thoracic Duct, Gabriel A. Inchauspe and Alfredo Rigalli Tracheostomy, Laura L. Pera and Alfredo Rigalli Section IV: Gastrointestinal Tract In Situ Isolation of the Stomach, Alfredo Rigalli In Situ Isolation of the Intestinal Loop, Maria L. Brance, Lucas R.M. Brun, and Alfredo Rigalli Intestinal Everted, Lucas R. M. Brun, Maria L. Brance, Lucas F. de Candia, and Alfredo Rigalli In Situ Perfusion of the Small Intestine, Alfredo Rigalli Section V: Pancreas Experimental Models for the Study of Diabetes, Veronica Di Loreto and Alfredo Rigalli Islets of Langerhans Isolation, Alfredo Rigalli Incubation of Pancreatic Tissue Slices, Ines Menoyo and Alfredo Rigalli In Situ Perfusion of The Pancreas, Ines Menoyo and Alfredo Rigalli Experimental Pancreatitis, Diego Holotte, Jesica Nipoti, Maria J. Pretini, Veronica Di Loreto, and Alfredo Rigalli Section VI: Liver Hepatic Circulation Impairment, Gabriel A. Inchauspe and Alfredo Rigalli Extrahepatic Cholestasis Model, Anabel Brandoni and Adriana M. Torres Section VII: Ablation of Endocrine Glands Adrenalectomy, Laura Pera and Alfredo Rigalli Ovariectomy, Veronica Di Loreto, Laura Pera, and Alfredo Rigalli Thyroparathyroidectomy, Veronica Di Loreto and Alfredo Rigalli Parathyroid Glands, Maela Lupo and Alfredo Rigalli Section VIII: Kidneys Measurement Techniques of Renal Parameters, Veronica Di Loreto and Alfredo Rigalli Kidney Isolation and Perfusion, Veronica Di Loreto and Alfredo Rigalli Acute Renal Failure Models, Gisela Di Giusto and Adriana M. Torres Renal Blood Flow Measurement, Gisela Di Giusto, Adriana M. Torres, and Alfredo Rigalli Transport Studies in Plasma Membrane Vesicles Isolated from Renal Cortex, Gisela Di Giusto and Adriana M. Torres Remnant Kidney Model, Maria V. Arcidiacono, L. A. Ramirez, V. Dalmau, and Alfredo Rigalli Section IX: Circulatory System Experimental Myocardial Infarction, Ignacio Seropian and German E. Gonzalez Experimental Arteriosclerosis, Anabel Brandoni and Adriana M. Torres Isolated Heart: Langendorff Technique, Bruno Buchholz, Veronica D'Annunzio, and Ricardo J. Gelpi Section X: Additional Techniques Polyclonal Antibodies Preparation, Laura I. Pera, Lucas R. M. Brun, and Alfredo Rigalli Histological Procedures, Maria L. Brance and Lucas R. M. Brun Bones and Bone Tissue, Hilda Moreno, Mercedes Lombarte, and Veronica Di Loreto Section XI: Miscellaneous Equipment, Alfredo Rigalli Solutions Preparation, Veronica Di Loreto and Alfredo Rigalli Normal Values In Iim/Fm Rat Subline "M", Veronica Di Loreto and Alfredo Rigalli Abbreviations


PRODUCT DETAILS

ISBN-13: 9781420093278
Publisher: Taylor & Francis (CRC Press Inc)
Publication date: May, 2009
Pages: 232
Dimensions: 178.00 x 254.00 x 23.00

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