The clinical microbiology laboratory is often a sentinel for the detection of drug resistant strains of microorganisms. Standardized protocols require continual scrutiny to detect emerging phenotypic resistance patterns. The timely notification of clinicians with susceptibility results can initiate the alteration of antimicrobial chemotherapy and improve patient care. It is vital that microbiology laboratories stay current with standard and emerging methods and have a solid understanding of their function in the war on infectious diseases."Antimicrobial Susceptibility Testing Protocols" clearly defines the role of the clinical microbiology laboratory in integrated patient care and provides a comprehensive, up-to-date procedural manual that can be used by a wide variety of laboratorians. The authors provide a comprehensive, up-to-date procedural manual including protocols for bioassay methods and molecular methods for bacterial strain typing. Divided into three sections, the text begins by introducing basic susceptibility disciplines including disk diffusion, macro and micro broth dilution, agar dilution, and the gradient method.
It covers step-by-step protocols with an emphasis on optimizing the detection of resistant microorganisms.The second section describes specialized susceptibility protocols such as surveillance procedures for detection of antibiotic-resistant bacteria, serum bactericidal assays, time-kill curves, population analysis, and synergy testing. The final section is designed to be used as a reference resource. Chapters cover antibiotic development; design and use of an antibiogram; and the interactions of the clinical microbiology laboratory with the hospital pharmacy, and infectious disease and control. Unique in its scope, "Antimicrobial Susceptibility Testing Protocols" gives laboratory personnel an integrated resource for updated lab-based techniques and charts within the contextual role of clinical microbiology in modern medicine.
An Overview of the Clinical and Laboratory Standards Institute and its Impact on Antimicrobial Susceptibility Testing, A. L. Barry Antimicrobial Classifications: Drugs for Bugs, C.B. Calderon, and B. Perdue Sabundayo Disk Diffusion Tests and Gradient Methodologies, A. Wanger Macro and Microdilution Methods of Antimicrobial Susceptibility Testing, S. Qaiyumi Automated Systems: An Overview, S. Stuckey Agar Dilution Susceptibility Testing, A. Hanlon, M. Taylor, and J. Dick Antibiograms/Preventive Surveillance: A Continuum of Data Collection, Analysis, and Presentation, J. Thomas and N.T. Rector Anaerobe Susceptibility Testing, D. E. Roe-Carpenter Antifungal Susceptibility Testing of Yeasts, A. Espinel-Ingroff and E. Canton Antifungal Susceptibility Testing of Filamentous Fungi, A. Espinel-Ingroff and E. Canton Susceptibility Testing of Mycobacteria, B. A. Brown-Elliott, S. Cohen, and R.J. Wallace, Jr. Methods for Determining Bactericidal Activity and Antimicrobial Interactions: Synergy Testing/Time-Kill Curves/Population Analysis, P. Verma Serum Bactericidal Testing, H. Nadler and M. Dowzicky Bioassay Methods for Antimicrobial and Antifungal Agents, D.H. Pitkin and E. Martin-Mazuelos Molecular Methods for Bacterial Strain Typing, S. Michaud and D. Berg Pharmacy and Microbiology: Interactive Synergy, B. Perdue Sabundayo and C.B. Calderon Interactions Between Clinicians and the Microbiology Laboratory, J.H. Powers Clinical Microbiology in the Development of New Antimicrobial Agents, C.D.Webb and B.G. Painter