Developmental biologists have been driven to investigate growth factor signaling in embryos in order to understand the regulatory mechanisms underlying a given developmental process. Thus, it is critical to explore the technical methods and experimental designs for growth factor signaling in embryos. Focusing on specific pathways or pathway components, "Analysis of Growth Factor Signaling in Embryos" provides the methods and guidelines for experimental design to study major aspects of cell signaling in vertebrate embryos. The book covers a broad range of topics in signaling and a variety of current model organisms.Section I explores specific signaling pathways or pathway components. In this section, some chapters highlight the biochemistry of signaling pathways during development, which is often distinctive from that observed in cell culture systems. Section II discusses ionic regulatory mechanisms and the two chapters in Section III examine ways of investigating gene regulation in response to extracellular signals. Finally, Section IV addresses emerging strategies that facilitate integrated analysis of cell signaling in vivo in embryonic systems.
Featuring contributions from expert researchers, "Analysis of Growth Factor Signaling in Embryos" will provide a foundation for further explorations of the cellular regulatory mechanisms governing vertebrate embryonic development.
SIGNALS AND PATHWAYS Analysis of Canonical Wnt Signaling in Xenopus Embryos Wilson K. Clements and David Kimelman How to Assay Non-Canonical Wnt Signaling: A Critical Analysis Michael Kuhl and Randall T. Moon Regulation of TGF-beta Family Activity by Proprotein Processing Shailaja Sopory and Jan L. Christian Analysis of MAP Kinase Pathways in Vertebrate Development Amy K. Sater and Heithem M. El-Hodiri Analysis of Retinoid Signalling in Embryos Malcolm Maden Wnt Signaling via the Rho Family of GTPases during Embryonic Development Raymond Habas and Xi He Phosphospecific Antibodies as Tools for the Study of Signal Transduction during Development Malcolm Whitman IONIC SIGNALS Dynamic Analysis of Calcium Signaling in Animal Development Diane C. Slusarski Strategies and Techniques for Investigation of Biophysical Signals in Patterning D.S. Adams and M. Levin TRANSCRIPTIONAL REGULATION OF TARGET GENES Expression Profiling in Xenopus Embryos Curtis Altmann Chromatin Immunoprecipitation for in vivo Studies of Transcriptional Regulation during Development Daniel R. Buchholz, Bindu D. Paul, and Yun-Bo Shi EMERGING STRATEGIES FOR THE ANALYSIS OF SIGNALING IN DEVELOPMENT Chemical Biology in Zebrafish Vascular Development Joanne Chan and Thomas M. Roberts Investigating Gastrulation Karen Symes Bringing Small Molecule Regulation of Protein Activity to Developmental Systems Karen J. Liu, Jason E. Gestwicki, and Gerald R. Crabtree Systems Analysis of Signaling Pathways Gregory R. Hoffman, Kevin Brown, Adrian Salic, and Ethan Lee INDEX