Main description:

This detailed book provides methodological information on cardiac gene delivery, from classic to state-of-the-art technologies and techniques. Efficient, cardiac-specific, and safe vectors, as well as refined vector delivery methods, are key for successful cardiac gene transfer and eventually for improving patients' outcomes. Newer vectors and more efficient vector delivery methods have the potential to dramatically improve gene transduction efficacy, while novel gene manipulation techniques enforce the therapeutic power and broaden disease targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Cardiac Gene Therapy: Methods and Protocols serves as a valuable tool for molecular biologists and physiologists in the cardiology field conducting cardiac gene transfer research, which will ultimately lead to further advancements in the vital field.

Contents:

Part I: Overview

1. Current Methods in Cardiac Gene Therapy: Overview

Kiyotake Ishikawa and Roger J. Hajjar

Part II: Genetic Modification

2. Silencing Genes in the Heart

Henry Fechner, Roland Vetter, Jens Kurreck, and Wolfgang Poller

3. Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs

Jimeen Yoo, Roger J. Hajjar, and Dongtak Jeong

4. Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro

Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, and Ioannis Karakikes

5. Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction

Hong Ma, Li Wang, Jiandong Liu, and Li Qian

Part III: Gene Delivery Vectors

6. Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9

Erik Kohlbrenner and Thomas Weber

7. Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV)

Andreas Jungmann, Oliver Muller, and Kleopatra Rapti

8. Synthesis of Modified mRNA for Myocardial Delivery

Jason Kondrat, Nishat Sultana, and Lior Zangi

9. Exosomes-Based Gene Therapy for MicroRNA Delivery

Prabhu Mathiyalagan and Susmita Sahoo

10. Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents

Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, and Kevin D. Costa

Part IV: Gene Delivery Methods

11. Gene Transfer in Isolated Adult Cardiomyocytes

Kjetil Hodne, David B. Lipsett, and William E. Louch

12. Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells

Francesca Stillitano, Ioannis Karakikes, and Roger J. Hajjar

13. Gene Transfer to Rodent Hearts In Vivo

Federica del Monte, Kiyotake Ishikawa, and Roger J. Hajjar

14. Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery

Shuyuan Chen and Paul A. Grayburn

15. A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy

Anthony S. Fargnoli, Michael G. Katz, and Charles R. Bridges

16. Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques

Shin Watanabe, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa

17. Direct Myocardial Injection of Vectors

Guillaume Bonnet, Kiyotake Ishikawa, Roger J. Hajjar, and Yoshiaki Kawase

18. Selective Pressure-Regulate Retroinfusion for Gene Therapy Application in Ischemic Heart Disease

Rabea Hinkel and Christian Kupatt

19. Cardiac Gene Delivery Using Recirculating Devices

Melissa J. Byrne and David M. Kaye

20. Molecular Cardiac Surgery with Recirculating Delivery (MCARD): Procedure and Vector Transfer

Michael G. Katz, Anthony S. Fargnoli, Andrew P. Kendle, and Charles R. Bridges

Part V: Targeting Arrhythmia

21. Gene Delivery for the Generation of Bioartificial Pacemaker

Patrick K.W. Chan and Ronald A. Li

22. Gene Therapy for Post-Infarction Ventricular Tachycardia

J. Kevin Donahue

Part VI: Targeting Pulmonary Hypertension

23. MicroRNA Delivery Strategies to the Lung in a Model of Pulmonary Hypertension

Lin Deng, Andrew H. Baker, and Angela C. Bradshaw

24. Inhaled Gene Transfer for Pulmonary Circulation

Jaume Aguero, Lahouaria Hadri, Nadjib Hammoudi, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa