Main description:

This volume details protocols that broadly cover many aspects of basic and translational research on Borrelia burgdorferi. Chapters guide readers through epidemiology and ecology, cultivation, cell structure, physiology, genomics and transcriptomics, proteomics, animal infection, pathogenesis and host responses, and vaccines. These essential protocols incorporate the most recent, practical, and innovative research tools aiding new and experienced researchers in their studies involving the biology, pathogenesis, and prevention of B. burgdorferi infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Borrelia burgdorferi: Methods and Protocols aims to ensure successful results in the further study of this vital field of biomedical research.

Contents:

1. Genotyping Strains of Lyme Disease Agents Directly From Ticks, Blood, or Tissue

Alan G. Barbour and Vanessa J. Cook

2. Species Identification and Phylogenetic Analysis of Borrelia burgdorferi Sensu Lato using Molecular Biological Methods

Gabriele Margos, Isabell Notter, and Volker Fingerle

3. Generation of Mammalian Host-adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats

Melissa J. Caimano

4. Co-immunoprecipitation for Identifying Protein-protein Interactions in Borrelia Burgdorferi

Henna Iqbal, Darrin R. Akins, and Melisha R. Kenedy

5. Characterization of Borrelia burgdorferi Binding to Mammalian Cells and Extracellular Matrix

Yi-Pin Lin and John M. Leong

6. Analysis of Lipids and Lipid Rafts in Borrelia

Alvaro Toledo, Zhen Huang, Jorge Benach, and Erwin London

7. Trace Element AnalysisofBorrelia burgdorferi by Inductively Coupled Plasma-Sector Field Mass Spectrometry

Xin Li, Peng Wang, Anthony Lutton, and John Olesik

8. Identification and Characterization of Borrelia burgdorferiComplement-binding Proteins

Peter Kraiczy

9. Artificial Infection of Ticks with Borrelia burgdorferi using a Microinjection Method and their Detection in vivo using Quantitative PCR Targeting flaB RNA

Alexis A. Smith, Xiuli Yang, Erol Fikrig, and Utpal Pal

10. Borrelia burgdorferi Microarray Analysis

Radha Iyer and Ira Schwartz

11. Borrelia burgdorferi Transcriptome Analysis by RNA-sequencing

Lybecker M and Henderson KC

12. Selection of Borrelia burgdorferi Promoter Sequences Active during Mammalian Infection Using In Vivo Expression Technology

Philip P. Adams and Mollie W. Jewett

13. Analysis of DNA and RNA Binding Properties of Borrelia burgdorferi Regulatory Proteins

J. Seshu , Trever C. Smith II, Ying-Han Lin, S.L. Rajasekhar Karna, Christine L. Miller, and Tricia Van Laar

14. Identification of Acetylated Proteins in Borrelia burgdorferi

Youyun Yang, Alan Wolfe, and X. Frank Yang

15. Genetic Transformation and Complementation

D. Scott Samuels, Dan Drecktrah, and Laura S. Hall

16. Genome-wide Mutagenesis in Borrelia burgdorferi

Tao Lin and Lihui Gao

17. Generation of Conditional Mutants in Borrelia burgdorferi

Jacob I. Latham and Jon S. Blevins

18. Detection of Bioluminescent Borrelia burgdorferi from in vitro

Jenny A. Hyde and Jon T. Skare

19. Analysis of Borrelia burgdorferi Proteome and Protein-protein Interactions

Xiuli Yang, Meghna Thakur, Juraj Koci, Alexis A. Smith, Preeti Singh, Xuran Zhuang, Kamoltip Promnares, Yan Wang, Ozlem Buyuktanir, and Utpal Pal

20. Two Photon Intravital Microscopy of Lyme Borrelia in Mice

Alexia A. Belperron, Jialing Mao, and Linda K. Bockenstedt

21. Investigating Human Dendritic Cell Immune Responses to Borrelia burgdorferi

Lauren M.K. Mason and Joppe W.R. Hovius

22. Phagocytosis Assays for Borrelia burgdorferi

Juan Anguita, Ana Carreras-Gonzalez, and Nicolas Navasa

23. Measuring Borrelia burgdorferi Motility and Chemotaxis

Kai Zhang and Chunhao Li

24. In vitro Models of Cutaneous Inflammation

Quentin Bernard, Benoit Jaulhac, and Nathalie Boulanger

25. Magnetic Isolation of Phagosomes Containing Toll like Receptor Ligands

Tanja Petnicki-Ocwieja and Linden T. Hu

26. Xenodiagnosis using Ixodes scapularis Larval Ticks in Humans

Siu-Ping Turk, Carla Williams, and Adriana Marques

27. Recombinant E. coli Dualistic Role as an Antigen-adjuvant Delivery Vehicle for Oral Immunization

Maria Gomes-Solecki and Luciana Richer