Main description:

This volume presents techniques and recent developments in biochemical approaches to study glutamatergic neurotransmission. This book contains detailed discussions on tracing neuronal pathways, functional or spectroscopic imaging, optogenetic or pharmacological tools, and extracellular neurochemistry in experimental clinical models. The chapters cover topics such as: identification of glutamatergic neurons in a discrete brain area, tools to study dynamics of single ionotropic receptors, in vivo extracellular glutamate monitoring in preclinical research using biosensors, capillary electrophoresis and liquid chromatography, and potential brain glutamatergic clinical biomarkers in biofluids and tissues. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Biochemical Approaches for Glutamatergic Neurotransmission is a valuable resource for researchers studying glutamate neurochemistry.

Contents:

Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1. Identification of Glutamatergic Neurons
Shiliang Zhang and Marisela Morales

2. Single Nanoparticle Tracking: A Method for Investigating the Surface Dynamics of Glutamate Receptors
Julien Dupuis, Francois Maingret, and Laurent Groc

3. Positron Emission Tomography of Metabotropic Glutamate Receptors
Selena Milicevic Sephton

4. NMR Spectroscopy of Brain Glutamate Function
Jun Shen

5. Imaging Glutamate with Genetically Encoded Fluorescent Sensors
Gerard J. Broussard, Elizabeth K. Unger, Ruqiang Liang, Brian P. McGrew, and Lin Tian

6. Measurement of Astrocytic Glutamate Release using Genetically-Encoded Probe Combined with TIRF Microscopy
Fuyuko Takata-Tsuji, Naura Choulnamountri, Christophe Place, and Olivier Pascual
7. Drugs to Alter Extracellular Concentration of Glutamate: Modulators of Glutamate Uptake Systems
Andreia Cristina Karklin Fontana

8. Drugs to Tune Up Glutamatergic Systems: Modulators of Glutamate Metabotropic Receptors
Kathy Sengmany and Karen J. Gregory

9. Glutamatergic Synthesis, Recycling, and Receptor Pharmacology at Drosophila and Crustacean Neuromuscular Junctions
Joshua S. Titlow and Robin L. Cooper

10. Optical Control of Glutamate Receptors of the NMDA-Kind in Mammalian Neurons, with the use of Photoswitchable Ligands
Shai Berlin and Ehud Y. Isacoff

11. In Vivo Electrochemical Studies of Optogenetic-Control of Glutamate Signaling Measured Using Enzyme-Based Ceramic Microelectrode Arrays
Jason J. Burmeister, Francois Pomerleau, Jorge E. Quintero, Peter Huettl, Yi Ai, Johan Jakobsson, Martin Lundblad, Andreas Heuer, John T. Slevin, and Greg A. Gerhardt

12. Separation-Based Sensors using Microchip Electrophoresis with Microdialysis for Monitoring Glutamate and Other Bioactive Amines
Nathan Oborny, Michael Hogard, and Susan Lunte

13. Brain Glutamate Monitoring by Microdialysis and Separation Methods with a Special Focus on Capillary Electrophoresis with Laser-Induced Fluorescence Detection
Luc Denoroy and Sandrine Parrot

14. In Vivo Determination of Glutamate Uptake by Brain Microdialysis
Sandrine Parrot, Monique Touret, and Luc Denoroy

15. Enzymes of Glutamate System
Irina S. Boksha, Olga K. Savushkina, Elena B. Tereshkina, Tatyana A. Prokhorova, and Elizabeta B. Mukaetova-Ladinska

16. The Glutamatergic System as Potential Clinical Biomarkers for Blood and Cerebrospinal Fluid Monitoring
Kenji Hashimoto

17. Clinical CNS Microdialysis of Glutamate with a Special Methodological Focus on Human Spinal Cord
Bernard Renaud, Luc Denoroy, Delphine Collin-Chavagnac, Patrick Mertens, and Sandrine Parrot