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MORE ABOUT THIS BOOK
Main description:
This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria. Beginning with protocols for the structural analysis of bacterial multidrug exporters, the book continues with sections on biochemical and bioengineering analysis, computational analysis, biomedical approaches, as well as advanced technologies expected to be applied to multidrug efflux transport studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Bacterial Multidrug Exporters: Methods and Protocols serves as an ideal guide to
this fast moving field of study.
Contents:
Part I: Structural Analysis of Bacterial Multidrug Exporters
1. High Resolution Crystallographic Analysis of AcrB Using Designed Ankyrin Repeat Proteins (DARPins)
Heng Keat Tam, Viveka Nand Malviya, and Klaas M. Pos
2. Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter AcrB in Physiologically-Relevant Asymmetric Crystals
Ryosuke Nakashima, Keisuke Sakurai, and Akihito Yamaguchi
3. Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitors
Tsukasa Kusakizako, Yoshiki Tanaka, Christopher J. Hipolito, Hiroaki Suga, and Osamu Nureki
4. Crystallographic Analysis of the CusBA Heavy-Me
tal Efflux Complex of Escherichia coli
Jared A. Delmar and Edward W. Yu
5. Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis
Dijun Du, Zhao Wang, Wah Chiu, and Ben F. Luisi
6. NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exporter EmrE
Maureen Leninger and Nathaniel J. Traaseth
7. Generation of Conformation-Specific Antibody Fragments for Crystallization of the Multidrug Resistance Transporter MdfA
Frank Jaenecke, Yoshiko Nakada-Nakura, Kumar Nagarathinam, Satoshi Ogasawara, Kehong Liu, Yunhon Hotta, So Iwata, Nor
imichi Nomura, and Mikio Tanabe
Part II: Biochemical and Bioengineering Analysis of Bacterial Multidrug Exporters
8. Biochemical Reconstitution and Characterization of Multi-Component Drug Efflux Transporters
Martin Picard, Elena B. Tikhonova, Isabelle Broutin, Shuo Lu, Alice Verchere, and Helen I. Zgurskaya
9. Covalently Linked Trimers of RND (Resistance-Nodulation-Division) Efflux Transporters to Study Their Mechanism of Action: Escherichia coli AcrB Multidrug Exporter as an Example
Hiroshi Nikaido
10. Determining Ligand Path through a Major Drug Transporter, AcrB, in Escherichia coli
Fasahath Husain and Hiroshi Nikaido
Part III: Computational Analysis of Bacterial Multidrug Exporters
11. Molecular Modeling of Multi-Drug Properties of Resistance Nodulation Division (RND) Transporters
Pierpaolo Cacciotto, V. K. Ramaswamy, Giuliano Malloci, Paolo Ruggerone, and Attilio V. Vargiu
12. A Transcriptomic Approach to Identify Novel Drug Efflux Pumps in Bacteria
Liping Li, Sasha G. Tetu, Ian T. Paulsen, and Karl A. Hassan
Part IV: Biomedical Approach for Bacterial Multidrug Exporters
13. Regulation of the Expression of Bacterial Multidrug Exporters by Two-Component Signal Transduction Systems
Kunihiko Nishino
14. Study of the Expression of Bacterial Multidrug Efflux Pumps in Anaerobic Conditions
Jingjing Sun, Ziqing Deng, Danny Ka Chun Fung, and Aixin Yan
15. Identification of a Staphylococcus aureus Efflux Pump Regulator Using a DNA-Protein Affinity Technique
Que Chi Truong-Bolduc and David C. Hooper
16. High Throughput Flow Cytometry Screening of Multidrug Efflux Systems
Mark K. Haynes, Matthew Garcia, Ryan Peters, Anna Waller, Pietro Tedesco, Oleg Ursu, Cristian G. Bologa, Radleigh G. Santos, Clemencia Pinilla, Terry H. Wu, Julie A. Lovchik, Tudor I. Oprea, Larry A. Sklar, and George P. Tegos
Part V: Advanced Technologies Expected for Application to Multi
drug Efflux Transport Studies
17. Single-Molecule Analysis of Membrane Transporter Activity by Means of a Microsystem
Rikiya Watanabe, Naoki Soga, Shin-ya Ohdate, and Hiroyuki Noji
18. Large Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria
Ryota Iino, Shouichi Sakakihara, Yoshimi Matsumoto, and Kunihiko Nishino
19. Reconstitution and Transport Analysis of Eukaryotic Transporters in the Post-Genomic Era
Hiroshi Omote and Yoshinori Moriyama
PRODUCT DETAILS
Publisher: Springer (Humana Press Inc.)
Publication date: November, 2017
Pages: None
Weight: 8342g
Availability: Available
Subcategories: Immunology