Main description:

A Structure-Function Toolbox for Membrane Transporter and Channels, Volume 594, a new release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET on transporters, Dynamics of channels and transporters investigated by NMR, Structure-function studies on channels and transporters, and a section on MemStar, a new GFP-based expression and purification system for transporters and channels.

Contents:

1. Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM 2. SSM-Based Electrophysiology for Transporter Research 3. Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters 4. Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy 5. The MEMbrane Protein Single ShoT Amplification Recipe: MemStar 6. Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli 7. Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling 8. Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR 9. Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein